What Is An SUV And LUV And How Do They Differ?

SUV are “small, unilamellar vesicles” or “Sonicated, Unilamellar Vesicles” and are usually prepared by sonication using a cuphorn, bath, or probe tip sonicator. LUV are “Large, Unilamellar Vesicles” and can be prepared by a variety of methods including extrusion (LUVET or “Large, Unilamellar Vesicles prepared by Extrusion Technique”), detergent dialysis (DOV or “Di-Octylglucoside Vesicles), fusion of SUV (FUV or “Fused, Unilamellar Vesicles”), reverse evaporation (REV or “Reverse Evaporation Vesicles), and ethanol injection. Unilamellar vesicles are prepared from MLV or LMV (Large, Multilamellar Vesicles), the large “onion-like” structures formed when amphiphilic lipids are hydrated. SUV are typically 15-30nm in diameter while LUV range from 100-200nm or larger. LUV are stable on storage, however, SUV will spontaneously fuse when they drop below the phase transition temperature of the lipid forming the vesicle.