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Procedure for TLC of synthetic PIPs

  1. Prepare 5% aqueous solution of potassium oxalate
  2. Dip TLC plate in potassium oxalate solution
  3. Dry and activate the TLC plate
  4. Lightly spot the plate with lipid; do not apply too much lipid to plate as it will cause streaking.
  5. Develop plate in chloroform:methanol:water:acetic acid (10:10:3:1, v/v)
  6. Visualize spots with suitable detection system, e.g., iodine vapor or molybdate spray
  7. Rf of PIPs (these values are reference points; actual results may vary depending on activity of plate and environmental conditions):
    • PIP2: 0.3-0.4
    • PIP3: ~0.2