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Tech Support
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- Liposome Preparation
- Lipid-DNA Preparation (Transfection)
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Analytical Procedures
- Determination of Total Phosphorus
- Thin Layer Chromatography (TLC) Analysis of Phospholipids
- Procedure for TLC of synthetic PIPs
- TLC Solvent Systems – Lipid Migration
- Detection Methods for Thin Layer Chromatography
- Fatty Acid Analysis of Phospholipids by GC/FID
- Determination of Phospholipid Oxidation by UV/VIS Spectroscopy
- Fatty Acid Composition
- Physical Properties
- Contract Manufacturing & Packaging
- Lipid Extraction
- External Resources
Procedure for TLC of synthetic PIPs
- Prepare 5% aqueous solution of potassium oxalate
- Dip TLC plate in potassium oxalate solution
- Dry and activate the TLC plate
- Lightly spot the plate with lipid; do not apply too much lipid to plate as it will cause streaking.
- Develop plate in chloroform:methanol:water:acetic acid (10:10:3:1, v/v)
- Visualize spots with suitable detection system, e.g., iodine vapor or molybdate spray
- Rf of PIPs (these values are reference points; actual results may vary depending on activity of plate and environmental conditions):
- PIP2: 0.3-0.4
- PIP3: ~0.2