850538 | Facade®-TEG

3a,7a,12a-tri-((O-b-D-glucopyranosyl)ethyloxy)-cholane


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25mg 850538P-25mg 850538P-25mg 1 x 25mg $171.75
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Facade®-TEG

Facade®-TEG

3a,7a,12a-tri-((O-b-D-glucopyranosyl)ethyloxy)-cholane

Façade detergents have a low micelle aggregation number, micelle mass, and hydrodynamic radius. Compared to conventional long-chain detergents, Facade-TEG facilitates the removal of free ligand partitioned into the micelle. This phenomenon resulted in lower background radioactivity for the structural analysis of type II cannabinoid receptor (CB2). Commonly used detergents such as DDM or CHAPS are not readily compatible with GEF assays to assess thermostability, but Façade-TEG forms small micelles and is better suited to study interactions of CB2 with G protein.

Beckner, R.; Zoubak, L.; Hines, K.; Gawrisch, K.; Yeliseev, A. Probing thermostability of detergent-solubilized CB2 receptor by parallel G protein-activation and ligand-binding assays. J. Biol. Chem. (2020) 295, 181-190. 

Hygroscopic
Yes
Light Sensitive
No
Molecular Formula
C48H84O21
Percent Composition
C 57.82%, H 8.49%, O 33.69%
Purity
>99%
Stability
1 Years
Storage Temperature
-20°C
CAS Number
1429405-97-9
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
997.168
Exact Mass
996.551
Synonyms
FA-5

Beckner RL, Zoubak L, Hines KG, Gawrisch K, Yeliseev AA. Probing thermostability of detergent-solubilized CB2 receptor by parallel G protein-activation and ligand-binding assays. J Biol Chem. 2020 Jan 3;295(1):181-190. doi: 10.1074/jbc.RA119.010696. Epub 2019 Nov 27. PMID: 31776188; PMCID: PMC6952600.

PubMed ID: 31776188

Lee SC, Bennett BC, Hong WX, Fu Y, Baker KA, Marcoux J, Robinson CV, Ward AB, Halpert JR, Stevens RC, Stout CD, Yeager MJ, Zhang Q. Steroid-based facial amphiphiles for stabilization and crystallization of membrane proteins. Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):E1203-11. doi: 10.1073/pnas.1221442110. Epub 2013 Mar 11. PMID: 23479627; PMCID: PMC3612649.

PubMed ID: 23479627

Zhang Q, Ma X, Ward A, Hong WX, Jaakola VP, Stevens RC, Finn MG, Chang G. Designing facial amphiphiles for the stabilization of integral membrane proteins. Angew Chem Int Ed Engl. 2007;46(37):7023-5. doi: 10.1002/anie.200701556. PMID: 17691085.

PubMed ID: 17691085