850500 | CHAPS

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

CHAPS

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CHAPS

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry. Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS’ small micellar molecular weight (6,150; aggregation number = 10) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.
Data
Hygroscopic
No
Light Sensitive
No
Molecular Formula
C32H58N2O7S
Percent Composition
C 62.51%, H 9.51%, N 4.56%, O 18.21%, S 5.21%
Purity
>99%
Stability
1 Years
Storage Temperature
-20°C
CAS Number
75621-03-3
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
614.877
Exact Mass
614.396
CMC
6 mM (20-25°C)
References

Kot EF, Arseniev AS, Mineev KS. On the behavior of most widely spread lipids in isotropic bicelles. Langmuir. 2018 Jun 20. doi: 10.1021/acs.langmuir.8b01454. [Epub ahead of print]

PubMed ID: 29924628

Cordwell, S.J. (2008) Sequential extraction of proteins by chemical reagents. Methods Mol Biol. 424:139-46.

PubMed ID: 18369859

Kot EF, Goncharuk SA, Arseniev AS, Mineev KS. Phase Transitions in Small Isotropic Bicelles. Langmuir. 2018 Mar 6. doi: 10.1021/acs.langmuir.7b03610.

PubMed ID: 29486112
Certificates of Analysis

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