Visit Our New YouTube Channel for Helpful Tutorials and Answers to Common Technical Questions.

Products

07:0 PC (DHPC) | 850306

1,2-diheptanoyl-sn-glycero-3-phosphocholine

850306P

Powder

25 mg
$86.00
200 mg
$155.00
500 mg
$285.00
1 g
$430.00

850306C

Chloroform

25 mg
$71.00
200 mg
$140.00
500 mg
$270.00
1 g
$415.00

All Prices in US Dollars
Custom packaging is calculated at $4.00 per aliquot and will be charged at the time of shipping.

Custom Packaging: Before clicking add to cart, from the drop down arrow select custom packaging. Next, edit the quantities (and concentration for solutions) information. Finally, click add to cart.

  • Product
  • Data
  • Downloads
  • Reference
The list of Phosphatidylcholine products offered by Avanti is designed to provide compounds having a variety of physical properties. Products available include short chain (C3-C8 are water soluble and hygroscopic), saturated, multi-unsaturated and mixed acid PC's. All of the products are purified by HPLC, and special precautions are taken to protect the products for oxidization and hydrolysis. Several of these products are manufactured under the current guidelines of Good Manufacturing Practice and are available for pharmaceutical use. If you have a requirement for a choline derivative not found on our list, please call us: custom synthesis is one of our specialties.
 
Preserve the Activity of Solubilized Membrane Proteins
 
Protein Preservation
DHPC preserves the native conformation and therefore the activity of the solubilized proteins. The three dimensional structure and activity of proteins are retained not only at the 10-15mM DHPC concentrations at which maximum solubilization usually occurs, but also over a large range of DHPC concentrations (up to 40mM). Not only is the three dimensional structure and hence the activity of most protein retained in excess DHPC, but the proteins also appear to be stable in DHPC. Such a result suggests that the direct interaction of DHPC with integral membrane proteins is weak. If the interaction of DHPC with membrane proteins is indeed weak, it follows that intrinsic membrane lipids will remain associated with the membrane proteins.
 
Solubilization Mechanism
DHPC is thought to exert a wedge-like effect on the neighboring lipids, mainly due to its bulky polar group and short hydrocarbon chains. This produces membrane destabilization at relatively low DHPC concentrations. All membranes investigated are solubilized at an identical DHPC / lipid ratio. This finding is taken as evidence that DHPC primarily interacts with the lipid bilayer and not with the membrane proteins. The principle underlying the preservation of the native protein structure is the inability of DHPC to displace intrinsic membrane lipids from integral membrane proteins.
 
Advantages of DHPC
  • DHPC preserves the activity of solubilized membrane proteins.
  • DHPC is not readily oxidized, and is stable over a wide pH range (4-10).
  • DHPC forms micelles rather than bilayers when dispersed in water. (CMC = 1.4mM)
  • DHPC shows a broad size distribution depending on the NaCl concentration of suspension.
  • DHPC does not interfere with spectrophotometric measurements.
  • DHPC is an ultra pure compound.
Molecular Formula
C22H44NO8P
Percent Composition
C 54.87%, H 9.21%, N 2.91%, O 26.58%, P 6.43
Purity
>99%
Stability
1 Years
Storage
-20°C
CAS Number
39036-04-9
CAS Registry Number is a Registered Trademark of the American Chemical Society
Molecular Weight
481.560
Exact Mass
481.280
CMC
1.4mM
Synonyms
DHPC
PC(7:0/7:0)
Download
Notes
GIF Graphics File. 
GIF Graphics File. 
MDL Molfile. 
CDX File. 
Hauser, H. (2000). Short-chain phospholipids as detergents. Biochim Biophys Acta 1508:164-81. [PubMed]



Kessi, J.,Poiree, J.C, Wehrli, E.,Bachofen, R.,Semenza, G.&Hauser, H.(1994). Short-chain phosphatidylcholines as superior detergents in solubilizing membrane proteins and preserving biological activity. Biochemistry 33: 10825-10836. [PubMed]