850306 | 07:0 PC (DHPC)

1,2-diheptanoyl-sn-glycero-3-phosphocholine


Powder

Size SKU Packaging Price
25mg 850306P-25mg 850306P-25mg 1 x 25mg $90.00
200mg 850306P-200mg 850306P-200mg 1 x 200mg $164.00
500mg 850306P-500mg 850306P-500mg 1 x 500mg $300.00
1g 850306P-1g 850306P-1g 1 x 1g $450.00

Chloroform

Size SKU Packaging Price
200mg 850306C-200mg 850306C-200mg 2 x 100mg 25mg/mL 4mL $164.00
500mg 850306C-500mg 850306C-500mg 1 x 500mg 25mg/mL 20mL $300.00
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07:0 PC (DHPC)

07:0 PC (DHPC)

1,2-diheptanoyl-sn-glycero-3-phosphocholine

The list of Phosphatidylcholine products offered by Avanti is designed to provide compounds having a variety of physical properties. Products available include short chain (C3-C8 are water soluble and hygroscopic), saturated, multi-unsaturated and mixed acid PC's. All of the products are purified by HPLC, and special precautions are taken to protect the products for oxidization and hydrolysis. Several of these products are manufactured under the current guidelines of Good Manufacturing Practice and are available for pharmaceutical use. If you have a requirement for a choline derivative not found on our list, please call us: custom synthesis is one of our specialties.
Preserve the Activity of Solubilized Membrane Proteins
Protein Preservation DHPC preserves the native conformation and therefore the activity of the solubilized proteins. The three dimensional structure and activity of proteins are retained not only at the 10-15mM DHPC concentrations at which maximum solubilization usually occurs, but also over a large range of DHPC concentrations (up to 40mM). Not only is the three dimensional structure and hence the activity of most protein retained in excess DHPC, but the proteins also appear to be stable in DHPC. Such a result suggests that the direct interaction of DHPC with integral membrane proteins is weak. If the interaction of DHPC with membrane proteins is indeed weak, it follows that intrinsic membrane lipids will remain associated with the membrane proteins.
Solubilization Mechanism DHPC is thought to exert a wedge-like effect on the neighboring lipids, mainly due to its bulky polar group and short hydrocarbon chains. This produces membrane destabilization at relatively low DHPC concentrations. All membranes investigated are solubilized at an identical DHPC / lipid ratio. This finding is taken as evidence that DHPC primarily interacts with the lipid bilayer and not with the membrane proteins. The principle underlying the preservation of the native protein structure is the inability of DHPC to displace intrinsic membrane lipids from integral membrane proteins.
Advantages of DHPC
  • DHPC preserves the activity of solubilized membrane proteins.
  • DHPC is not readily oxidized, and is stable over a wide pH range (4-10).
  • DHPC forms micelles rather than bilayers when dispersed in water. (CMC = 1.4mM)
  • DHPC shows a broad size distribution depending on the NaCl concentration of suspension.
  • DHPC does not interfere with spectrophotometric measurements.
  • DHPC is an ultra pure compound.
Hygroscopic
Yes
Light Sensitive
No
Molecular Formula
C22H44NO8P
Percent Composition
C 54.87%, H 9.21%, N 2.91%, O 26.58%, P 6.43
Purity
>99%
Stability
1 Years
Storage Temperature
-20°C
CAS Number
39036-04-9
CAS Registry Number is a Registered Trademark of the American Chemical Society
Molecular Weight
481.560
Exact Mass
481.280
Synonyms
<p>DHPCPC(7:0/7:0)</p>

Kot EF, Arseniev AS, Mineev KS. On the behavior of most widely spread lipids in isotropic bicelles. Langmuir. 2018 Jun 20. doi: 10.1021/acs.langmuir.8b01454. [Epub ahead of print]

PubMed ID: 29924628

Hauser, H. (2000). Short-chain phospholipids as detergents. Biochim Biophys Acta 1508:164-81. [PubMed]

PubMed ID: 11090824

Kessi, J.,Poiree, J.C, Wehrli, E.,Bachofen, R.,Semenza, G.&Hauser, H.(1994). Short-chain phosphatidylcholines as superior detergents in solubilizing membrane proteins and preserving biological activity. Biochemistry 33: 10825-10836. [PubMed]

PubMed ID: 8075085