810600 | 16:0-12 Doxyl PC



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1mg 810600P-1mg 810600P-1mg 1 x 1mg $559.65
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16:0-12 Doxyl PC

16:0-12 Doxyl PC


Avanti’s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins [Smirnova et al, 2007] and as fluorescent quenchers in lipid bilayer structural studies [Kondo et al, 2008].
Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies [Wu and Gaffney, 2006]. For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 µl absolute ethanol for a concentration of 40.3 mM [Kondo et al, 2008, supplemental info found at http://pubs.acs.org/doi/suppl/10.1021/ja804929m/suppl_file/ja804929m_si_001.pdf].
Light Sensitive
Molecular Formula
Percent Composition
C 64.08%, H 10.52%, N 3.25%, O 18.56%, P 3.59%
1 Years
Storage Temperature
CAS Number
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
Exact Mass

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PubMed ID: 31836519

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PubMed ID: 31836521

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PubMed ID: 31378067

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PubMed ID: 31004798

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PubMed ID: 14718921

Kay, J.G., M. Koivusalo, X. Ma, T. Wohland, and S. Grinstein. (2012). Phosphatidylserine dynamics in cellular membranes. Mol Biol Cell 23:2198-212.

PubMed ID: 22496416

Kondo M, Mehiri M, Regen SL. (2008) Viewing membrane-bound molecular umbrellas by parallax analyses. J Am Chem Soc. 130:13771-7.

PubMed ID: 18783220

Smirnova TI, Chadwick TG, Voinov MA, Poluektov O, van Tol J, Ozarowski A,Schaaf G, Ryan MM, Bankaitis VA. (2007) Local polarity and hydrogen bonding inside the Sec14p phospholipid-binding cavity: high-field multi-frequency electron paramagnetic resonance studies. Biophys J. 92:3686-95.

PubMed ID: 17325006

Wu F, Gaffney BJ. (2006) Dynamic behavior of fatty acid spin labels within a binding site of soybean lipoxygenase-1. Biochemistry 45:12510-8.

PubMed ID: 17029406

Alaouie AM, Smirnov AI. (2006) Ultra-stable temperature control in EPR experiments: thermodynamics of gel-to-liquid phase transition in spin-labeled phospholipid bilayers and bilayer perturbations by spin labels. J Magn Reson. 182:229-38.

PubMed ID: 16859937

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PubMed ID: 8908298

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PubMed ID: 2159806

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PubMed ID: 3210953

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PubMed ID: 7378080

Ausili A, Torrecillas A, deGodos AM, Corbalan-Garcia S, Gomez-Fernandez JC. The phenolic group of α-tocopherol anchors in the lipid-water interface of fully saturated membranes. Langmuir. 2018 Feb 15. doi: 10.1021/acs.langmuir.7b04142.

PubMed ID: 29447442