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TopFluor®-POVPE (DMA) | 810406

1-palmitoyl-2-(5,5-dimethoxyvaleroyl)-sn-glycero-3-phosphoethanolamine-N-
[4-(dipyrrometheneboron difluoride)butanoyl] (ammonium salt)

810406M

Methylene Chloride

1 mg
$340.00
5 mg
$1020.00
10 mg
$1600.00

All Prices in US Dollars
Custom packaging is calculated at $4.00 per aliquot and will be charged at the time of shipping.

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  • Data
  • Downloads
  • Reference
  • Deprotection Procedure
  • Spectrum
Molecular Formula
C43H72BF2N4O10P
Percent Composition
C 58.06%, H 8.45%, B 1.16%, F 4.08%, N 6.02%, O 18.91%, P 3.33%
Purity
>99%
Stability
1 Years
Storage
-20°C
Molecular Weight
930.903
Exact Mass
930.547
Ex/Em
497/511nm
Download
Notes
GIF Graphics File. 
GIF Graphics File. 
MDL Molfile. 
CDX File. 
Moumtzi, A., M. Trenker, K. Flicker, E. Zenzmaier, R. Saf, and A. Hermetter. (2007). Import and fate of fluorescent analogs of oxidized phospholipids in vascular smooth muscle cells. J Lipid Res 48:565-82. [PubMed]
Note: Perform all operations in a fume hood.
 
(1) Open glass ampoule and transfer the contents of the ampoule to the Teflon vial (included) using a glass pipette or syringe. DO NOT USE PLASTIC.
(2) Evaporate the dichloromethane under a gentle stream of nitrogen at room temperature.
(3) Add 250µL of 200mM HCl in THF (see below for preparation) and stir/agitate for 1 hour.
(4) Neutralize the reaction mixture with 10mg of NaHCO3 solid
(5) Extract the aldehyde product with 900µL of CHCl3/MeOH ( 2:1, v/v).
(6) Wash the resulting organic phase twice with 250 µL of water
(7) Dry the organic phase over Na2SO4, filter, and evaporate organic solvent under a gentle stream of nitrogen at room temperature. This procedure will yield ~1mg of aldehyde product.
(8)  Once deprotected, we recommend that TopFluor POVPE be used immediately.
 
Preparation of 200mM HCl in THF: 2mL of 1N aq HCl was diluted with 8mL of THF.