810184 | TopFluor® PI(4,5)P2

1-oleoyl-2-{6-[4-(dipyrrometheneboron difluoride)butanoyl]amino}hexanoyl-sn-glycero-3-phosphoinositol-4,5-bisphosphate (ammonium salt)


Powder

Size SKU Packaging Price
50µg 810184P-50ug 810184P-50ug $552.00
Base Price: ${originalprice|money}
Custom Packaging: (${concentration} @$4.00/ea. + $100) ${custompackagingtotal|money}
Packaging: ${concentration}
Item Total: ${totalprice|money}
(Sales Tax may apply)
Please select an option above.
${sku} - ${concentration}

TopFluor® PI(4,5)P<SUB>2</SUB>

TopFluor® PI(4,5)P2

1-oleoyl-2-{6-[4-(dipyrrometheneboron difluoride)butanoyl]amino}hexanoyl-sn-glycero-3-phosphoinositol-4,5-bisphosphate (ammonium salt)

Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT.
Hygroscopic
No
Light Sensitive
No
Molecular Formula
C50H92N6O20P3BF2
Percent Composition
C 48.47%, H 7.48%, B 0.87%, F 3.07%, N 6.78%, O 25.83%, P 7.50%
Purity
>99%
Stability
1 Year
Storage Temperature
-20°C
CAS Number
1246355-57-6
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
1239.023
Exact Mass
1238.564

Sun S, Liu C, Rodriguez Melendez D, Yang T, Cremer PS. Immobilization of Phosphatidylinositides Revealed by Bilayer Leaflet Decoupling. J Am Chem Soc. 2020 Jul 20. doi: 10.1021/jacs.0c03800. Epub ahead of print. PMID: 32687699.

PubMed ID: 32687699

Bousova K, Barvik I, Herman P, Hofbauerová K, Monincova L, Majer P, Zouharova M, Vetyskova V, Postulkova K, Vondrasek J. Mapping of CaM, S100A1 and PIP2-Binding Epitopes in the Intracellular N- and C-Termini of TRPM4. Int J Mol Sci. 2020 Jun 17;21(12):E4323. doi: 10.3390/ijms21124323. PMID: 32560560.

PubMed ID: 32560560

Yoshioka D, Fukushima S, Koteishi H, Okuno D, Ide T, Matsuoka S, Ueda M. Single-molecule imaging of PI(4,5)P2 and PTEN in vitro reveals a positive feedback mechanism for PTEN membrane binding. Commun Biol. 2020 Feb 28;3(1):92. doi: 10.1038/s42003-020-0818-3. PMID: 32111929; PMCID: PMC7048775.

PubMed ID: 32111929

Shi, X., M. Kohram, X. Zhuang, and A.W. Smith. (2016). Interactions and Translational Dynamics of Phosphatidylinositol Bisphosphate (PIP) Lipids in Asymmetric Lipid Bilayers. Langmuir [PubMed]

PubMed ID: 26829708

Maria J. Sarmento, Ana Coutinho, Aleksander Fedorov, Manuel Prieto, and Fabio Fernandes. Membrane Order Is a Key Regulator of Divalent Cation-Induced Clustering of PI(3,5)P2 and PI(4,5)P2. Langmuir. Article ASAP. [PubMed]

PubMed ID: 28961003
TopFluorEmEx4of.gif