Products

12-NBD Stearate | 810110

12-N-methyl-(7-nitrobenz-2-oxa-1,3-diazo)aminostearic acid

810110P

Powder

1 mg
$185.00
5 mg
$295.00
10 mg
$420.00

810110C

Chloroform

1 mg
$170.00
5 mg
$280.00
10 mg
$405.00

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  • Product
  • Data
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  • Binding Info
  • Cell Images
NBD-stearate is a fluorescent fatty acid analogue used to probe fatty acid binding protein (FABP) and sterol carrier protein-2 (SCP-2).
Molecular Formula
C25H40N4O5
Percent Composition
C 63.00%, H 8.46%, N 11.76%, O 16.78
Purity
>99%
Stability
6 Months
Storage
-20°C
CAS Number
117056-67-4
CAS Registry Number is a Registered Trademark of the American Chemical Society
Molecular Weight
476.609
Exact Mass
476.300
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Notes
GIF Graphics File. 
GIF Graphics File. 
MDL Molfile. 
CDX File. 
Pohl J, Ring A, Stremmel W. Uptake of long-chain fatty acids in HepG2 cells involves caveolae: analysis of a novel pathway. J Lipid Res. 2002 Sep;43(9):1390-9. [PubMed]



Atshaves BP, Storey SM, McIntosh AL, Petrescu AD, Lyuksyutova OI, Greenberg AS, Schroeder F. Sterol carrier protein-2 expression modulates protein and lipid composition of lipid droplets. J Biol Chem. 2001 Jul 6;276(27):25324-35. [PubMed]



Milliano MT, Luxon BA. The peroxisomal proliferator clofibrate enhances the hepatic cytoplasmic movement of fatty acids in rats. Hepatology. 2001 Feb;33(2):413-8. [PubMed]



Murphy EJ. Sterol carrier protein-2 expression increases NBD-stearate uptake and cytoplasmic diffusion in L cells. Am J Physiol. 1998 Aug;275(2 Pt 1):G237-43. [PubMed]



Atshaves BP, Foxworth WB, Frolov A, Roths JB, Kier AB, Oetama BK, Piedrahita JA, Schroeder F. Cellular differentiation and I-FABP protein expression modulate fatty acid uptake and diffusion. Am J Physiol. 1998 Mar;274(3 Pt 1):C633-44. [PubMed]



Schroeder F, Myers-Payne SC, Billheimer JT, Wood WG. Probing the ligand binding sites of fatty acid and sterol carrier proteins: effects of ethanol. Biochemistry. 1995 Sep 19;34(37):11919-27. [PubMed]



Luxon BA. Inhibition of binding to fatty acid binding protein reduces the intracellular transport of fatty acids. Am J Physiol. 1996 Jul;271(1 Pt 1):G113-20. [PubMed]

 
 

Data generously provided by Dr. Huan Huang and Dr. Fred Schroeder, Texas A & M University.

Pattern of distribution in living L-cell fibroblasts

NBD-stearate was taken up by L-cells, was not toxic at the low concentrations used, and distributed diffusely throughout the cytoplasm (at low concentrations) and both in cytoplasm and brightly into lipid droplets (at higher concentrations).


300nM NBD-Stearate, 15min 500nM NBD-Stearate, 15min 20nM Nile Red, 15min

 

Photos courtesy of Dr. Huan Huang and Dr. Fred Schroeder, Texas A & M University.