790326 | Fluorescent Transfection Reagent

Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE


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10mg 790326P-10mg 790326P-10mg 1 x 10mg $451.50

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Fluorescent Transfection Reagent

Fluorescent Transfection Reagent, contains DOTAP:DOPE (1:1, w/w) + 0.1 wt.% NBD-DOPE

DOTAP is one of the most widely used cationic lipids for gene transfection applications. DOTAP is proven to be efficient for in vitro and in vivo transfection applications. Various analogues of TAP are available for structure-activity relationship studies.

Simberg D, Weisman S, Talmon Y, Barenholz Y. DOTAP (and other cationic lipids): chemistry, biophysics, and transfection. Crit Rev Ther Drug Carrier Syst. 2004;21(4):257-317. doi: 10.1615/critrevtherdrugcarriersyst.v21.i4.10. PMID: 15638468.

Data
Hygroscopic
Yes
Light Sensitive
Yes
Stability
1 Year
Storage Temperature
-20°C
References

Lewis RN, McElhaney RN. Surface charge markedly attenuates the nonlamellar phase-forming propensities of lipid bilayer membranes: calorimetric and (31)P-nuclear magnetic resonance studies of mixtures of cationic, anionic, and zwitterionic lipids. Biophys J. 2000 Sep;79(3):1455-64. doi: 10.1016/S0006-3495(00)76397-1. PMID: 10969007; PMCID: PMC1301039.

PubMed ID: 10969007

Wong GC, Tang JX, Lin A, Li Y, Janmey PA, Safinya CR. Hierarchical self-assembly of F-actin and cationic lipid complexes: stacked three-layer tubule networks. Science. 2000 Jun 16;288(5473):2035-9. doi: 10.1126/science.288.5473.2035. PMID: 10856215.

PubMed ID: 10856215

Macdonald PM, Crowell KJ, Franzin CM, Mitrakos P, Semchyschyn D. 2H NMR and polyelectrolyte-induced domains in lipid bilayers. Solid State Nucl Magn Reson. 2000 May;16(1-2):21-36. doi: 10.1016/s0926-2040(00)00051-5. PMID: 10811426.

PubMed ID: 10811426

Kim TW, Chung H, Kwon IC, Sung HC, Jeong SY. In vivo gene transfer to the mouse nasal cavity mucosa using a stable cationic lipid emulsion. Mol Cells. 2000 Apr 30;10(2):142-7. doi: 10.1007/s10059-000-0142-1. PMID: 10850654.

PubMed ID: 10850654

Meidan VM, Cohen JS, Amariglio N, Hirsch-Lerner D, Barenholz Y. Interaction of oligonucleotides with cationic lipids: the relationship between electrostatics, hydration and state of aggregation. Biochim Biophys Acta. 2000 Apr 5;1464(2):251-61. doi: 10.1016/s0005-2736(00)00151-6. PMID: 10727612.

PubMed ID: 10727612

Simões S, Slepushkin V, Pires P, Gaspar R, Pedroso de Lima MC, Düzgüneş N. Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum. Biochim Biophys Acta. 2000 Feb 15;1463(2):459-69. doi: 10.1016/s0005-2736(99)00238-2. PMID: 10675522.

PubMed ID: 10675522
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