699852 | 3D-PHAD®

Monophosphoryl 3-Deacyl Lipid A (Synthetic)Pat No. 9,241,988


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Monophosphoryl 3-Deacyl Lipid A (Synthetic)Pat No. 9,241,988

Vaccination is well-accepted as an effective method to prevent infections by mounting pathogen-specific immune responses prior to the infection. Usually, immunization with vaccine antigens alone is not able to induce robust or long-lasting immune responses — resulting in failure of protective immunity against infections. Thus, adjuvants are required to enhance cellular or humoral immune responses upon immunization. Because vaccine adjuvants using Lipid A have proven to be safe and effective in inducing Th-1 type immune responses to heterologous proteins in animal and human vaccines, Avanti developed Phosphorylated HexaAcyl Disaccharide (PHAD®), the first fully synthetic monophosphoryl Lipid A available for use as an adjuvant in human vaccines.

The highly pure MPLA analog, 3D-PHAD®, provides a homogeneous synthetic equivalent for the 3-deacylated MPLA derived from bacterial LPS. While comparable to bacterial MPLA and other synthetic MPLA analogs at eliciting an immune response in a liposomal adjuvant system (see bar graph), 3D-PHAD® is less pyrogenic than its bacterial-derived mimic. Extensive preclinical testing with 3D-PHAD® demonstrated equivalency to PHAD®, and human trials have been scheduled for launch. 3D-PHAD® is protected under Pat No. 9,241,988. Licensing opportunities are available for vaccine or immunotherapy commercialization.

Stimulatory effect of PHAD®, 3D-PHAD®, and 3D(6-acyl)-PHAD® on macrophages. Macrophage cell line J774 cells were cultured with Avanti PHAD®, 3D-PHAD®, or 3D(6-acyl)-PHAD® for 24hrs. IL-12 levels in supernatants were measured by sandwich ELISA.


             Adjuvant Activity

Antigen: gp140 from HIV-1

PHAD®, 3D-PHAD®, and 3D(6A)-PHAD® have been tested extensively in animals using a variety of antigens. In all cases, these adjuvants exhibit a similar activity and safety profile to bacterially-derived MPL. The data above demonstrate the equivalency of the three synthetic adjuvants to the bacterially-derived MPL when presented in a liposomal carrier system (DMPC/DMPG/Cholesterol).

Light Sensitive
Molecular Formula
Percent Composition
C 64.07%, H 10.36%, N 2.73%, O 20.82%, P 2.02%
1 Years
Storage Temperature
CAS Number
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
Exact Mass

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PubMed ID: 32703436

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PubMed ID: 31775762

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PubMed ID: 31567352

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PubMed ID: 31446807

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PubMed ID: 31176851

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PubMed ID: 31043512

Cawlfield A, Genito CJ, Beck Z, Bergmann-Leitner ES, Bitzer AA, Soto K, Zou X, Hadiwidjojo SH, Gerbasi RV, Mullins AB, Noe A, Waters NC, Alving CR, Matyas GR, Dutta S. Safety, toxicity and immunogenicity of a malaria vaccine based on the circumsporozoite protein (FMP013) with the adjuvant army liposome formulation containing QS21 (ALFQ). Vaccine. 2019 Jun 27;37(29):3793-3803. doi: 10.1016/j.vaccine.2019.05.059. Epub 2019 May 28.

PubMed ID: 31151801

Taleghani N, Bozorg A, Azimi A, Zamani H. Immunogenicity of HPV and HBV vaccines: adjuvanticity of synthetic analogs of monophosphoryl lipid A combined with aluminum hydroxide. APMIS. 2019 Mar;127(3):150-157. doi: 10.1111/apm.12927.

PubMed ID: 30746792