699800 | MPLA (PHAD®)

Monophosphoryl Lipid A (Synthetic) (PHAD®)

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MPLA (PHAD®)

MPLA (PHAD®)

Monophosphoryl Lipid A (Synthetic) (PHAD®)

Vaccination is well-accepted as an effective method to prevent infections by mounting pathogen-specific immune responses prior to the infection. Usually, immunization with vaccine antigens alone is not able to induce robust or long-lasting immune responses — resulting in failure of protective immunity against infections. Thus, adjuvants are required to enhance cellular or humoral immune responses upon immunization. Because vaccine adjuvants using Lipid A have proven to be safe and effective in inducing Th-1 type immune responses to heterologous proteins in animal and human vaccines, Avanti developed Phosphorylated HexaAcyl Disaccharide (PHAD®), the first fully synthetic monophosphoryl Lipid A available for use as an adjuvant in human vaccines.


PHAD® is a synthetic structural analog of monophosphoryl Lipid A (MPLA) that has been shown to boost the immune system through activation of the toll-like receptor 4 (TLR4) resulting in production of proinflammatory cytokines and antigen-specific effector CD4+ and memory CD8+ T cells. Also referred to as GLA, this adjuvant has been administered to well over 1000 human subjects without serious adverse events. PHAD® is available in bulk quantities for vaccine development and commercial manufacturing.


Stimulatory effect of PHAD®, 3D-PHAD®, and 3D(6-acyl)-PHAD® on macrophages. Macrophage cell line J774 cells were cultured with Avanti PHAD®, 3D-PHAD®, or 3D(6-acyl)-PHAD® for 24hrs. IL-12 levels in supernatants were measured by sandwich ELISA.

            

             Adjuvant Activity

Antigen: gp140 from HIV-1












PHAD®, 3D-PHAD®, and 3D(6A)-PHAD® have been tested extensively in animals using a variety of antigens. In all cases, these adjuvants exhibit a similar activity and safety profile to bacterially-derived MPL. The data above demonstrate the equivalency of the three synthetic adjuvants to the bacterially-derived MPL when presented in a liposomal carrier system (DMPC/DMPG/Cholesterol).

Hygroscopic
No
Light Sensitive
No
Molecular Formula
C96H184N3O22P
Percent Composition
C 65.38%, H 10.52%, N 2.38% ,O 19.96%, P 1.76
Purity
> 99%
Stability
1 Years
Storage Temperature
-20°C
CAS Number
1246298-63-4
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
1763.469
Exact Mass
1762.311
Synonyms
PHAD®
phosphorylated hexaacyl disaccharide
Glycopyranoside Lipid A (GLA)

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Hernandez A, Luan L, Stothers CL, Patil NK, Fults JB, Fensterheim BA, Guo Y, Wang J, Sherwood ER, Bohannon JK. Phosphorylated Hexa-Acyl Disaccharides Augment Host Resistance Against Common Nosocomial Pathogens. Crit Care Med. 2019 Nov;47(11):e930-e938. doi: 10.1097/CCM.0000000000003967.

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PubMed ID: 21349366

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 PubMed ID: 20593273