Aurora®-PLC | 550002

Aurora®-PLC Nanoparticles




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3-5nm gold nanoparticle stabilized with dodecanethiol, coated with 1-myristoyl-sn-glycero-3-phosphocholine
1 Year
Miao Li, Theobald Lohmüller, and Jochen Feldmann (2014).Optical Injection of Gold Nanoparticles into Living Cells. Nano Lett., Article ASAP DOI: 10.1021/nl504497m Publication Date (Web): December 11, 2014.The controlled injection of nanoscopic objects into living cells with light offers promising prospects for the development of novel molecular delivery strategies or intracellular biosensor applications. Here, we show that single gold nanoparticles from solution can be patterned on the surface of living cells with a continuous wave laser beam. In a second step, we demonstrate how the same particles can then be injected into the cells through a combination of plasmonic heating and optical force. We find that short exposure times are sufficient to perforate the cell membrane and inject the particles into cells with a survival rate of >70%. [Link]Allijn, I.E., W. Leong, J. Tang, A. Gianella, A.J. Mieszawska, F. Fay, G. Ma, S. Russell, C.B. Callo, R.E. Gordon, E. Korkmaz, J.A. Post, Y. Zhao, H.C. Gerritsen, A. Thran, R. Proksa, H. Daerr, G. Storm, V. Fuster, E.A. Fisher, Z.A. Fayad, W.J. Mulder, and D.P. Cormode. (2013). Gold nanocrystal labeling allows low-density lipoprotein imaging from the subcellular to macroscopic level. ACS Nano 7:9761-70. [PubMed]