Oxidized Phospholipid Snoopers® | 330501

Oxidized Phospholipid Snoopers®



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Avanti Ox-Phospholipid Snoopers consist of 12 different lipid species spotted individually on a solid support. Each spot contains 1 µg of the highest quality pure lipid and is ideal for investigating lipid-protein interactions.
This product should be stored at 4°
Not the perfect mix of lipids for your application? Avanti will prepare custom Snoopers tailored to your exact requirements. Contact a Product Specialist with your series of lipids and let us revolutionize your science.
1 Year
Bruntz, R.C., H.E. Taylor, C.W. Lindsley, and H.A. Brown. (2013). Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells. J Biol Chem [PubMed]Gallego, O., M.J. Betts, J. Gvozdenovic-Jeremic, K. Maeda, C. Matetzki, C. Aguilar-Gurrieri, P. Beltran-Alvarez, S. Bonn, C. Fernandez-Tornero, L.J. Jensen, M. Kuhn, J. Trott, V. Rybin, C.W. Muller, P. Bork, M. Kaksonen, R.B. Russell, and A.C. Gavin. (2010). A systematic screen for protein-lipid interactions in Saccharomyces cerevisiae. Mol Syst Biol 6:430. [PubMed]
Avanti Snoopers have been used as a method to determine/confirm the specificity of Avanti’s E06 antibody, which recognizes the phosphocholine headgroup of oxidized phospholipid that is present in oxidized LDL. E06 does not bind to unoxidized phosphocholine or normal LDL.
Ox-Phospholipid Snoopers were blocked with 3% BSA (fatty acid free) in TBS (0.8% NaCl, 20 mM Tris-HCL pH 7.4). The blocked membranes were then probed with E06 (200 ng/mL) in TBS containing 1% BSA for
1 h at RT. After washing the membranes 3 times with TBS containing
1 mM EDTA the bound antibody was detected with goat antimouse IgM conjugated to horseradish peroxidase (HRP) (Southern Biotech) at a 1:10,000 dilution in TBS containing 1% BSA for 1 h at RT. After washing, bound HRP was visualized on an X-ray film with an enhanced chemiluminescence substrate (Pierce).