Inositol Snoopers® | 330500

Inositol Snoopers®



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Avanti Inositol Snoopers® consist of 13 different lipid species spotted individually on a solid support. Each spot contains 1 µg of the highest quality pure lipid and is ideal for investigating lipid-protein interactions.
This product should be stored at 4°
Not the perfect mix of lipids for your application? Avanti will prepare custom Snoopers tailored to your exact requirements. Contact a Product Specialist with your series of lipids and let us revolutionize your science.
1 Year
Bruntz, R.C., H.E. Taylor, C.W. Lindsley, and H.A. Brown. (2013). Phospholipase D2 mediates survival signaling through direct regulation of Akt in glioblastoma cells. J Biol Chem [PubMed]Gallego, O., M.J. Betts, J. Gvozdenovic-Jeremic, K. Maeda, C. Matetzki, C. Aguilar-Gurrieri, P. Beltran-Alvarez, S. Bonn, C. Fernandez-Tornero, L.J. Jensen, M. Kuhn, J. Trott, V. Rybin, C.W. Muller, P. Bork, M. Kaksonen, R.B. Russell, and A.C. Gavin. (2010). A systematic screen for protein-lipid interactions in Saccharomyces cerevisiae. Mol Syst Biol 6:430. [PubMed]
Avanti Snoopers have been used as a method to determine/confirm the specificity of Avanti’s WR304 antibody, which recognizes PIP and PIP2.
Inositol Snoopers were blocked with 3% BSA (fatty acid free) in TBS (0.8% NaCl, 20 mM Tris-HCL pH 7.4) containing 1 mM EDTA. The blocked membranes were then probed with WR304 (1.0 μg/mL) in TBS containing 1% BSA and 1 mM EDTA for 1 h at RT. After washing the membranes 3 times with TBS containing 1 mM EDTA the bound antibody was detected with goat antimouse IgM conjugated to horseradish peroxidase (HRP) (Southern Biotech) at a 1:10,000 dilution in TBS containing 1% BSA and 1 mM EDTA for 1 h at RT. After washing, bound HRP was visualized on an X-ray film with an enhanced chemiluminescence substrate (Pierce).