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WR304 mAb | 330021

WR304 Mouse Monoclonal Antibody (IgM)
Anti-(PIP/PIP2)

330021S

Saline

100 μg
$245.00

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  • Antibody Information
WR304 is an antibody that binds specifically to the lipids PIP and PIP2. WR304 also neutralizes infectious HIV-1 virus and can be used to probe the presence of PIP and PIP2 in membranes in a variety of applications including inositol phosphate-dependent cell signaling pathways.
 
This antibody was developed by the Walter Reed Army Institute of Research (WRAIR) using a targeted adjuvant protocol with liposomes containing Avanti’s Lipid A.
Purity
>95%
Stability
6 Months
Storage
The recommended long-term storage for WR304 is at -80°C. After initial thawing remaining product should refrozen at -80°C.
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Notes
Wassef, N.M., F. Roerdink, G.M. Swartz Jr, J.A. Lyon, B.J. Berson, and C.R. Alving. (1984). Phosphate-binding specificities of monoclonal antibodies against phosphoinositides in liposomes. Mol Immunol 21:863-8. [PubMed]

Matyas, G.R., Z. Beck, N. Karasavvas, and C.R. Alving. (2009). Lipid binding properties of 4E10, 2F5, and WR304 monoclonal antibodies that neutralize HIV-1. Biochim Biophys Acta 1788:660-5. [PubMed]

Brown, B.K., N. Karasavvas, Z. Beck, G.R. Matyas, D.L. Birx, V.R. Polonis, and C.R. Alving. (2007). Monoclonal antibodies to phosphatidylinositol phosphate neutralize human immunodeficiency virus type 1: role of phosphate-binding subsites. J Virol 81:2087-91. [PubMed]

Beck, Z., N. Karasavvas, J. Tong, G.R. Matyas, M. Rao, and C.R. Alving. (2007). Calcium modulation of monoclonal antibody binding to phosphatidylinositol phosphate. Biochem Biophys Res Commun 354:747-51. [PubMed]

Antigens: Brain PI(4)P.
Ig Class: Mouse IgM (kappa).
Specificity: WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus.
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture.
Purification: Ultra filtration through 100 KDa cut-off filters.
Purity: ≥ 95%.
Formulation: WR304 is provided as a 0.2µm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.
Concentration: 1 mg/ml.
Recommended Applications: ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity.
Storage conditions: Store undiluted at either -20°C or -80°C.
Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.
 
Product use:
The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).