WR304 mAb | 330021

WR304 Mouse Monoclonal Antibody (IgM)




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WR304 is an antibody that binds specifically to the lipids PIP and PIP2. WR304 also neutralizes infectious HIV-1 virus and can be used to probe the presence of PIP and PIP2 in membranes in a variety of applications including inositol phosphate-dependent cell signaling pathways.
This antibody was developed by the Walter Reed Army Institute of Research (WRAIR) using a targeted adjuvant protocol with liposomes containing Avanti’s Lipid A.
1 Year
The recommended long-term storage for WR304 is at -80°C. After initial thawing remaining product should refrozen at -80°C.
Wassef, N.M., F. Roerdink, G.M. Swartz Jr, J.A. Lyon, B.J. Berson, and C.R. Alving. (1984). Phosphate-binding specificities of monoclonal antibodies against phosphoinositides in liposomes. Mol Immunol 21:863-8. [PubMed]Matyas, G.R., Z. Beck, N. Karasavvas, and C.R. Alving. (2009). Lipid binding properties of 4E10, 2F5, and WR304 monoclonal antibodies that neutralize HIV-1. Biochim Biophys Acta 1788:660-5. [PubMed]Brown, B.K., N. Karasavvas, Z. Beck, G.R. Matyas, D.L. Birx, V.R. Polonis, and C.R. Alving. (2007). Monoclonal antibodies to phosphatidylinositol phosphate neutralize human immunodeficiency virus type 1: role of phosphate-binding subsites. J Virol 81:2087-91. [PubMed]Beck, Z., N. Karasavvas, J. Tong, G.R. Matyas, M. Rao, and C.R. Alving. (2007). Calcium modulation of monoclonal antibody binding to phosphatidylinositol phosphate. Biochem Biophys Res Commun 354:747-51. [PubMed]
Antigens: Brain PI(4)P. Ig Class: Mouse IgM (kappa). Specificity: WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus. Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304. Production: In vitro cell culture. Purification: Ultra filtration through 100 KDa cut-off filters. Purity: ≥ 95%. Formulation: WR304 is provided as a 0.2µm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii. Concentration: 1 mg/ml. Recommended Applications: ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity. Storage conditions: Store undiluted at either -20°C or -80°C. Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.
Product use: The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).