WR304 mAb | 330021
WR304 Mouse Monoclonal Antibody (IgM)
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- Antibody Information
- 6 Months
- The recommended long-term storage for WR304 is at -80°C. After initial thawing remaining product should refrozen at -80°C.
Matyas, G.R., Z. Beck, N. Karasavvas, and C.R. Alving. (2009). Lipid binding properties of 4E10, 2F5, and WR304 monoclonal antibodies that neutralize HIV-1. Biochim Biophys Acta 1788:660-5. [PubMed]
Brown, B.K., N. Karasavvas, Z. Beck, G.R. Matyas, D.L. Birx, V.R. Polonis, and C.R. Alving. (2007). Monoclonal antibodies to phosphatidylinositol phosphate neutralize human immunodeficiency virus type 1: role of phosphate-binding subsites. J Virol 81:2087-91. [PubMed]
Beck, Z., N. Karasavvas, J. Tong, G.R. Matyas, M. Rao, and C.R. Alving. (2007). Calcium modulation of monoclonal antibody binding to phosphatidylinositol phosphate. Biochem Biophys Res Commun 354:747-51. [PubMed]
Ig Class: Mouse IgM (kappa).
Specificity: WR304 recognizes PIP and PIP2, and neutralizes HIV-1 virus.
Antibody Source: Monoclonal antibody from BALB/c-derived hybridoma WR304.
Production: In vitro cell culture.
Purification: Ultra filtration through 100 KDa cut-off filters.
Purity: ≥ 95%.
Formulation: WR304 is provided as a 0.2µm sterile-filtered solution in Tris buffered saline (TBS). WR304 binding to porcine brain PI(4)P is inhibited by high concentration of Ca2+ (10 mM)iv and addition of 1 mM EDTA stimulates this binding activity. PBS and other phosphate-containing buffers should not be used as they inhibit binding to the phospholipid head groupiii.
Concentration: 1 mg/ml.
Recommended Applications: ELISA, IHC, Dot Blot, Flow cytometry and HIV-1 infectivity.
Storage conditions: Store undiluted at either -20°C or -80°C.
Hazardous/Non-hazardous Components: This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore, there is no MSDS for this product.
The WR304 antibody has been used for the quantitation of PIP/PIP2 in direct ELISA and Dot Blot analysis. The WR304 antibody specifically binds to PIP/PIP2 and inhibits the infectivity of HIV-1 to peripheral blood mononuclear cells (PBMC).