330003 | E06 mAb-TopFluor®
E06 Mouse Monoclonal Antibody (IgM), TopFluor® labeledAnti-(Oxidized Phospholipid)
E06 mAb-TopFluor®
E06 Mouse Monoclonal Antibody (IgM), TopFluor® labeledAnti-(Oxidized Phospholipid)
Palinski W, Hörkkö S, Miller E, Steinbrecher UP, Powell HC, Curtiss LK, Witztum JL. Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest. 1996 Aug 1;98(3):800-14. doi: 10.1172/JCI118853. PMID: 8698873; PMCID: PMC507491.
PubMed ID: 8698873For binding studies with the E06-TopFluor™ antibody, 96-well Microfluor 1 black U bottom plates (Thermo 7005) were coated with 50µL of antigen (5µg/mL) in PBS containing 0.27 mM EDTA and overnight at 4°C. The wells were washed three times with PBS containing 0.27 mM EDTA. After washing, the plates were blocked with 3% BSA in PBS for 45 min at room temperature. 100µL aliquots of a serial dilution of the E06-TopFluor™ antibody (0.78-400ng/well) in PBS containing EDTA and 1% BSA were added to each well and incubated 1hr at room temperature. The amount of IgM bound to each well was quantified with after 1hr incubation at room temperature with excitation (Ex) at 485 nm and emission (Em) recorded at 520 nm.