Products

E06 mAb-biotinylated | 330002

E06 Mouse Monoclonal Antibody (IgM), Biotinylated
Anti-(Oxidized Phospholipid)

330002S

Saline

25 μg
$245.00

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  • Product Use
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples.
 
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques.

Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is verified for activity and provided with detailed protocols for the above applications.

The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
Purity
>95%
Stability
6 Months
Storage
We recommend long-term storage of E06 at -80°C. After it has been thawed for initial use, we recommend storing at 4°C. We do not recommend freeze-thawing or aliquoting.
Synonyms
T15 antibody
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Notes
Tsimikas, S., Aikawa, M., Miller, F.J., Jr., Miller, E.R., Torzewski, M., Lentz, S.R., Bergmark, C., Heistad, D.D., Libby, P., Witztum, J.L. (2007) Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vasc Biol. 27:175-81.

Tsimikas, S., Bergmark, C., Beyer, R.W., Patel, R., Pattison, J., Miller, E., Juliano, J., Witztum, J.L. (2003) Temporal increases in plasma markers of oxidized low-density lipoprotein strongly reflect the presence of acute coronary syndromes. J Am Coll Cardiol. 41:360-70.

Friedman, P., Hörkkö, S., Steinberg, D., Witztum, J.L., Dennis, E.A. (2002) Correlation of antiphospholipid antibody recognition with the structure of synthetic oxidized phospholipids. Importance of Schiff base formation and aldol condensation. J Biol Chem. 277:7010-20.

Hörkkö, S., Bird, D.A., Miller, E., Itabe, H., Leitinger, N., Subbanagounder, G., Berliner, J.A., Friedman, P., Dennis, E.A., Curtiss, L.K., Palinski, W., Witztum, J.L. (1999) Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins. J Clin Invest. 103:117-28.

Palinski, W., Hörkkö, S., Miller, E., Steinbrecher, U.P., Powell, H.C., Curtiss, L.K., Witztum, J.L. (1996) Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest. 98:800-14.

The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 µg/mL working solution of 12 µg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 µg/mL working solution (1:100 dilution).