330002 | E06 mAb-biotinylated
E06 Mouse Monoclonal Antibody (IgM), BiotinylatedAnti-(Oxidized Phospholipid)
E06 mAb-biotinylated
E06 Mouse Monoclonal Antibody (IgM), BiotinylatedAnti-(Oxidized Phospholipid)
- Certificate of Analysis (Lot No. 330002S-25UG-A-010 and 5022SBA010)
- Safety Data Sheet
- Technical Data Sheet
- Certificate of Analysis (Lot No. 330002S-25UG-A-012 and 5022SBA012)
Coliva G, Duarte S, Pérez-Sala D, Fedorova M. Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells. Redox Biol. 2019 Jan 24:101123. doi: 10.1016/j.redox.2019.101123. [Epub ahead of print]
PubMed ID: 30737170Tsimikas, S., Aikawa, M., Miller, F.J., Jr., Miller, E.R., Torzewski, M., Lentz, S.R., Bergmark, C., Heistad, D.D., Libby, P., Witztum, J.L. (2007) Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vasc Biol. 27:175-81.
Tsimikas, S., Bergmark, C., Beyer, R.W., Patel, R., Pattison, J., Miller, E., Juliano, J., Witztum, J.L. (2003) Temporal increases in plasma markers of oxidized low-density lipoprotein strongly reflect the presence of acute coronary syndromes. J Am Coll Cardiol. 41:360-70.
Friedman, P., Hörkkö, S., Steinberg, D., Witztum, J.L., Dennis, E.A. (2002) Correlation of antiphospholipid antibody recognition with the structure of synthetic oxidized phospholipids. Importance of Schiff base formation and aldol condensation. J Biol Chem. 277:7010-20.
Hörkkö, S., Bird, D.A., Miller, E., Itabe, H., Leitinger, N., Subbanagounder, G., Berliner, J.A., Friedman, P., Dennis, E.A., Curtiss, L.K., Palinski, W., Witztum, J.L. (1999) Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins. J Clin Invest. 103:117-28.
Palinski, W., Hörkkö, S., Miller, E., Steinbrecher, U.P., Powell, H.C., Curtiss, L.K., Witztum, J.L. (1996) Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest. 98:800-14.
The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 µg/mL working solution of 12 µg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 µg/mL working solution (1:100 dilution).