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E06 mAb | 330001

E06 Mouse Monoclonal Antibody (IgM)
Anti-(Oxidized Phospholipid)



100 μg

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  • Antibody Information
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques. Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is verified for activity and provided with detailed protocols for the above applications. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
1 year
We recommend long-term storage of E06 at -80°C. After it has been thawed for initial use, we recommend storing at 4°C. We do not recommend freeze-thawing or aliquoting.
T15 antibody
Chattopadhyay, A., Navab, M., Hough, G., Grijalva, V., Mukherjee, P., Fogelman, H.R., Hwang, L.H., Faull, K.F., Lusis, A.J., Reddy, S.T., Fogelman, A.M. (2016) Tomatoes expressing the 6F peptide ameliorate the increase in oxidized phospholipids in the jejunum of mice fed unsaturated lysophosphatidylcholine or western diet. J Lipid Res. Mar 9. [Pubmed]

Tsimikas, S., Aikawa, M., Miller, F.J., Jr., Miller, E.R., Torzewski, M., Lentz, S.R., Bergmark, C., Heistad, D.D., Libby, P., Witztum, J.L. (2007) Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vasc Biol. 27:175-81 [PubMed]

Qin, J., Goswami, R., Balabanov, R., Dawson, G. (2007) Oxidized phosphatidylcholine is a marker for neuroinflammation in multiple sclerosis brain. J Neurosci Res. 85:977-84. [PubMed]

Friedman, P., Hörkkö, S., Steinberg, D., Witztum, J.L., Dennis, E.A. (2002) Correlation of antiphospholipid antibody recognition with the structure of synthetic oxidized phospholipids. Importance of Schiff base formation and aldol condensation. J Biol Chem. 277:7010-20. [PubMed]

Hörkkö, S., Bird, D.A., Miller, E., Itabe, H., Leitinger, N., Subbanagounder, G., Berliner, J.A., Friedman, P., Dennis, E.A., Curtiss, L.K., Palinski, W., Witztum, J.L. (1999) Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins. J Clin Invest. 103:117-28. [PubMed]

Palinski, W., Hörkkö, S., Miller, E., Steinbrecher, U.P., Powell, H.C., Curtiss, L.K., Witztum, J.L. (1996) Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest.98: 800-14. [PubMed]
Oxidized LDL
Ig Class:
Mouse IgM (kappa)
E06 recognizes the phosphocholine headgroup of oxidized phospholipid that is present in oxidized LDL and PC-modified BSA. E06 does not bind to normal LDL or unoxidized PC.
Antibody Source:
Monoclonal antibody from C57BL/6-derived hybridoma E06.
In vitro cell culture.
Ultra filtration through 100 KDa cut-off filters.
≥ 95%.
E06 is provided as a 0.2μm sterile-filtered solution
in phosphate buffered saline (PBS).
ELISA (Sandwich, Direct & Competitive), IHC and WB (see Technical Data Sheet under Downloads tab for more information).
Storage conditions:
Store undiluted at 4°C or less.
Hazardous/Non-hazardous Components:
This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore there is no MSDS for this product.