330001 | E06 mAb

E06 Mouse Monoclonal Antibody (IgM)Anti-(Oxidized Phospholipid)

Aqueous

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100µg 330001S-100ug 330001S-100ug $291.00
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E06 mAb

E06 Mouse Monoclonal Antibody (IgM)Anti-(Oxidized Phospholipid)

The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques.

Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is
verified for activity and provided with detailed protocols for the above applications.

The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Hygroscopic
No
Light Sensitive
No
Purity
>95%
Stability
1 year
Storage Temperature
We recommend long-term storage of E06 at -80°C. After it has been thawed for initial use, we recommend storing at 4°C. We do not recommend freeze-thawing or aliquoting.
Formula Weight
0.000
Exact Mass
0.000
Synonyms
<p>T15 antibody</p>

Jayaraman S, Chavez OR, Pérez A, Miñambres I, Sánchez-Quesada JL, Gursky O. Binding to heparin triggers deleterious structural and biochemical changes in human low-density lipoprotein, which are amplified in hyperglycemia. Biochim Biophys Acta Mol Cell Biol Lipids. 2020 Apr 11;1865(8):158712. doi: 10.1016/j.bbalip.2020.158712. Epub ahead of print. PMID: 32289504.

 PubMed ID: 32289504

Sun X, Seidman JS, Zhao P, Troutman TD, Spann NJ, Que X, Zhou F, Liao Z, Pasillas M, Yang X, Magida JA, Kisseleva T, Brenner DA, Downes M, Evans RM, Saltiel AR, Tsimikas S, Glass CK, Witztum JL. Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic Steatohepatitis. Cell Metab. 2019 Nov 14. pii: S1550-4131(19)30605-9. doi: 10.1016/j.cmet.2019.10.014. [Epub ahead of print]

 PubMed ID: 31761566

Berger M, Raslan Z, Aburima A, Magwenzi S, Wraith KS, SPurgeon BEJ, Hindle MS, Law R, Febbraio M, Naseem KM. Atherogenic lipid stress induces platelet hyperactivity through CD36-mediated hyposensitivity to prostacyclin-; the role of phosphodiesterase 3A. Haematologica. 2019 Jul 9. pii: haematol.2018.213348. doi: 10.3324/haematol.2018.213348. [Epub ahead of print]

 PubMed ID: 31289200

Colombo S, Criscuolo A, Zeller M, Fedorova M, Domingues MR, Domingues P. Analysis of oxidised and glycated aminophospholipids: Complete structural characterisation by C30 liquid chromatography-high resolution tandem mass spectrometry. Free Radic Biol Med. 2019 May 28. pii: S0891-5849(19)30356-9. doi: 10.1016/j.freeradbiomed.2019.05.025. [Epub ahead of print]

 PubMed ID: 31141712

Sakai T, Imai J, Takagaki H, Ui M, Hatta S. Cytoplasmic OH scavenger TA293 attenuates cellular senescence and fibrosis by activating macrophages through oxidized phospholipids/TLR4. Life Sci. 2019 Mar 15;221:284-292. doi: 10.1016/j.lfs.2019.02.038. Epub 2019 Feb 19.

 PubMed ID: 30794829

Afonso CB, Spickett CM. Lipoproteins as targets and markers of lipoxidation. Redox Biol. 2018 Dec 6:101066. doi: 10.1016/j.redox.2018.101066. [Epub ahead of print]

 PubMed ID: 30579928

Que, Xuchu, Hung, Ming-Yow, Yeang, Calvin, Gonen, Ayelet, Prohaska, Thomas A., Sun, Xiaoli, Diehl, Cody, Määttä, Antti, Gaddis, Dalia E., Bowden, Karen, Pattison, Jennifer, MacDonald, Jeffrey G., Ylä-Herttuala, Seppo, Mellon, Pamela L., Hedrick, Catherine C., Ley, Klaus, Miller, Yury I., Glass, Christopher K., Peterson, Kirk L., Binder, Christoph J., Tsimikas, Sotirios, Witztum, Joseph L. Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. 2018 Nature Letter. Published 06 June 2018

NATURE

Chattopadhyay, A., Navab, M., Hough, G., Grijalva, V., Mukherjee, P., Fogelman, H.R., Hwang, L.H., Faull, K.F., Lusis, A.J., Reddy, S.T., Fogelman, A.M. (2016) Tomatoes expressing the 6F peptide ameliorate the increase in oxidized phospholipids in the jejunum of mice fed unsaturated lysophosphatidylcholine or western diet. J Lipid Res. Mar 9.

PubMed ID: 26965826

Tsimikas, S., Aikawa, M., Miller, F.J., Jr., Miller, E.R., Torzewski, M., Lentz, S.R., Bergmark, C., Heistad, D.D., Libby, P., Witztum, J.L. (2007) Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vasc Biol. 27:175-81

 PubMed ID: 17082490

Qin, J., Goswami, R., Balabanov, R., Dawson, G. (2007) Oxidized phosphatidylcholine is a marker for neuroinflammation in multiple sclerosis brain. J Neurosci Res. 85:977-84.

PubMed ID: 17304573

Friedman, P., Hörkkö, S., Steinberg, D., Witztum, J.L., Dennis, E.A. (2002) Correlation of antiphospholipid antibody recognition with the structure of synthetic oxidized phospholipids. Importance of Schiff base formation and aldol condensation. J Biol Chem. 277:7010-20.

 PubMed ID: 11744722

Hörkkö, S., Bird, D.A., Miller, E., Itabe, H., Leitinger, N., Subbanagounder, G., Berliner, J.A., Friedman, P., Dennis, E.A., Curtiss, L.K., Palinski, W., Witztum, J.L. (1999) Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins. J Clin Invest. 103:117-28.

PubMed ID: 9884341

Palinski, W., Hörkkö, S., Miller, E., Steinbrecher, U.P., Powell, H.C., Curtiss, L.K., Witztum, J.L. (1996) Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest.98: 800-14.

PubMed ID: 8698873
Antigen:
 Oxidized LDL
Ig Class:
 Mouse IgM (kappa)
Specificity:
 E06 recognizes the phosphocholine headgroup of oxidized phospholipid that is present in oxidized LDL and PC-modified BSA. E06 does not bind to normal LDL or unoxidized PC.
Antibody Source:
 Monoclonal antibody from C57BL/6-derived hybridoma E06.
Production:
 In vitro cell culture.
Purification:
Ultra filtration through 100 KDa cut-off filters.
Purity:
 ≥ 95%.
Formulation:
 E06 is provided as a 0.2μm sterile-filtered solution in phosphate buffered saline (PBS).
Recommended Applications:
ELISA (Sandwich, Direct & Competitive), IHC and WB (see Technical Data Sheet under Downloads tab for more information).
Storage conditions:
Store undiluted at 4°C or less.
Hazardous/Non-hazardous Components:
This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore there is no MSDS for this product.