330001 | E06 mAb
E06 Mouse Monoclonal Antibody (IgM)Anti-(Oxidized Phospholipid)
E06 mAb
E06 Mouse Monoclonal Antibody (IgM)Anti-(Oxidized Phospholipid)
The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques.
Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is
verified for activity and provided with detailed protocols for the above applications.
The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.
- Certificate of Analysis (Lot No. 330001S-100UG-A-019 and 5021SDA019)
- Certificate of Analysis (Lot No. 330001S-100UG-A-024 and 5021SDA024)
- Safety Data Sheet
- Technical Data Sheet
Que, Xuchu, Hung, Ming-Yow, Yeang, Calvin, Gonen, Ayelet, Prohaska, Thomas A., Sun, Xiaoli, Diehl, Cody, Määttä, Antti, Gaddis, Dalia E., Bowden, Karen, Pattison, Jennifer, MacDonald, Jeffrey G., Ylä-Herttuala, Seppo, Mellon, Pamela L., Hedrick, Catherine C., Ley, Klaus, Miller, Yury I., Glass, Christopher K., Peterson, Kirk L., Binder, Christoph J., Tsimikas, Sotirios, Witztum, Joseph L. Oxidized phospholipids are proinflammatory and proatherogenic in hypercholesterolaemic mice. 2018 Nature Letter. Published 06 June 2018
Chattopadhyay, A., Navab, M., Hough, G., Grijalva, V., Mukherjee, P., Fogelman, H.R., Hwang, L.H., Faull, K.F., Lusis, A.J., Reddy, S.T., Fogelman, A.M. (2016) Tomatoes expressing the 6F peptide ameliorate the increase in oxidized phospholipids in the jejunum of mice fed unsaturated lysophosphatidylcholine or western diet. J Lipid Res. Mar 9.
PubMed ID: 26965826Tsimikas, S., Aikawa, M., Miller, F.J., Jr., Miller, E.R., Torzewski, M., Lentz, S.R., Bergmark, C., Heistad, D.D., Libby, P., Witztum, J.L. (2007) Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vasc Biol. 27:175-81
PubMed ID: 17082490Qin, J., Goswami, R., Balabanov, R., Dawson, G. (2007) Oxidized phosphatidylcholine is a marker for neuroinflammation in multiple sclerosis brain. J Neurosci Res. 85:977-84.
PubMed ID: 17304573Friedman, P., Hörkkö, S., Steinberg, D., Witztum, J.L., Dennis, E.A. (2002) Correlation of antiphospholipid antibody recognition with the structure of synthetic oxidized phospholipids. Importance of Schiff base formation and aldol condensation. J Biol Chem. 277:7010-20.
PubMed ID: 11744722Hörkkö, S., Bird, D.A., Miller, E., Itabe, H., Leitinger, N., Subbanagounder, G., Berliner, J.A., Friedman, P., Dennis, E.A., Curtiss, L.K., Palinski, W., Witztum, J.L. (1999) Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins. J Clin Invest. 103:117-28.
PubMed ID: 9884341Palinski, W., Hörkkö, S., Miller, E., Steinbrecher, U.P., Powell, H.C., Curtiss, L.K., Witztum, J.L. (1996) Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest.98: 800-14.
PubMed ID: 8698873
Antigen: |
Oxidized LDL |
Ig Class: |
Mouse IgM (kappa) |
Specificity: |
E06 recognizes the phosphocholine headgroup of oxidized phospholipid that is present in oxidized LDL and PC-modified BSA. E06 does not bind to normal LDL or unoxidized PC. |
Antibody Source: |
Monoclonal antibody from C57BL/6-derived hybridoma E06. |
Production: |
In vitro cell culture. |
Purification: |
Ultra filtration through 100 KDa cut-off filters. |
Purity: |
≥ 95%. |
Formulation: |
E06 is provided as a 0.2μm sterile-filtered solution in phosphate buffered saline (PBS). |
Recommended
Applications: |
ELISA (Sandwich, Direct & Competitive), IHC and WB (see Technical Data Sheet under Downloads tab for more information). |
Storage conditions: |
Store undiluted at 4°C or less. |
Hazardous/Non-hazardous Components: |
This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore there is no MSDS for this product. |