300213 | LNP Lipid Blend 2
LNP Lipid Blend 2
LNP Lipid Blend 2
LNP Lipid Blend 2
This pre-mixed lipid blend is composed of components suitable for producing nucleic acid-based lipid nanoparticles. Works best with DNA/plasmid cargo, and can be used for other nucleic acids as well.
Instructions for using the LNP Lipid Blend 2:
1. Add 1-mL of ethanol to the vial to achieve 25 mg/mL. Mix well to achieve full dissolution.*
2. Dilute nucleic acid in aqueous buffer.**
3. Use solvent injection to create LNPs. Different parameters and cargo will result in different particle size results.
4. Dialyze the final formulation against buffer of choice to remove residual ethanol.
Catalog Number | Component Name | Mole Fraction |
890890 | DOTAP | 49 |
700107 | Sitosterol (synthetic from plant sterol) | 38.5 |
850365 | DSPC | 11 |
880151 | DMG-PEG2000 | 1.5 |
*Note: low heat, sonication, or vortexing can be used as needed
**Note: common buffers include citrate, Tris, or PBS