100600 | E. coli Extract Polar

E. coli Polar Lipid Extract


Powder

Size SKU Packaging Price
100mg 100600P-100mg 100600P-100mg 1 x 100mg $166.00
500mg 100600P-500mg 100600P-500mg 5 x 100mg $515.00
1g 100600P-1g 100600P-1g 1 x 1g $830.00

Chloroform

Size SKU Packaging Price
100mg 100600C-100mg 100600C-100mg 1 x 100mg 25mg/mL 4mL $166.00
500mg 100600C-500mg 100600C-500mg 5 x 100mg 25mg/mL 4mL $515.00
1g 100600C-1g 100600C-1g 2 x 500mg 25mg/mL 20mL $830.00
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E. coli Extract Polar

E. coli Polar Lipid Extract

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase.
 
Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.
Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.
Avanti does not perform any analytical procedures on this extract.
 
Hygroscopic
Yes
Light Sensitive
No
Stability
1 Year
Storage Temperature
-20°C
CAS Number
1240502-50-4
CAS Registry Number is a Registered Trademark of the American Chemical Society
Formula Weight
0.000
Exact Mass
0.000

Kumar A, Russell RM, Pifer R, Menezes-Garcia Z, Cuesta S, Narayanan S, MacMillan JB, Sperandio V. The Serotonin Neurotransmitter Modulates Virulence of Enteric Pathogens. Cell Host Microbe. 2020 Jul 8;28(1):41-53.e8. doi: 10.1016/j.chom.2020.05.004. Epub 2020 Jun 9. PMID: 32521224; PMCID: PMC7351610.

PubMed ID: 32521224

Chattrakun K, Hoogerheide DP, Mao C, Randall LL, King GM. Protein Translocation Activity in Surface-Supported Lipid Bilayers. Langmuir. 2019 Sep 17;35(37):12246-12256. doi: 10.1021/acs.langmuir.9b01928. Epub 2019 Sep 6. Erratum in: Langmuir. 2020 May 26;: PMID: 31448613.

PubMed ID: 31448613

Matin TR, Utjesanovic M, Sigdel KP, Smith VF, Kosztin I, King GM. Characterizing the Locus of a Peripheral Membrane Protein-Lipid Bilayer Interaction Underlying Protein Export Activity in E. coli. Langmuir. 2020 Mar 3;36(8):2143-2152. doi: 10.1021/acs.langmuir.9b03606. Epub 2020 Feb 18. PMID: 32011890.

PubMed ID: 32011890

Reddy B, Bavi N, Lu A, Park Y, Perozo E. Molecular basis of force-from-lipids gating in the mechanosensitive channel MscS. Elife. 2019 Dec 27;8:e50486. doi: 10.7554/eLife.50486. PMID: 31880537.

PubMed ID: 31880537

Liu S, Fukumoto T, Gena P, Feng P, Sun Q, Li Q, Matsumoto T, Kaneko T, Zhang H, Zhang Y, Zhong S, Zeng W, Katsuhara M, Kitagawa Y, Wang A, Calamita G, Ding X. Ectopic expression of a rice plasma membrane intrinsic protein (OsPIP1;3) promotes plant growth and water uptake. Plant J. 2019 Dec 23;10.1111/tpj.14662. doi: 10.1111/tpj.14662. [Epub ahead of print]. PMID: 31872463.

PubMed ID: 31872463

Teixeira-Duarte CM, Fonseca F, Morais-Cabral JH. Activation of a nucleotide-dependent RCK domain requires binding of a cation cofactor to a conserved site. Elife. 2019 Dec 23;8:e50661. doi: 10.7554/eLife.50661. PMID: 31868587; PMCID: PMC6957272.

PubMed ID: 31868587

Duša F, Chen W, Witos J, Rantamäki AH, King AWT, Sklavounos E, Roth M, Wiedmer SK. Immobilization of natural lipid biomembranes and their interactions with choline carboxylates. A nanoplasmonic sensing study. Biochim Biophys Acta Biomembr. 2020 Feb 1;1862(2):183115. doi: 10.1016/j.bbamem.2019.183115. Epub 2019 Nov 5.

PubMed ID: 31704086

Patiño-Ruiz M, Fendler K, Călinescu O. Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae. Sci Rep. 2019 Oct 28;9(1):15390. doi: 10.1038/s41598-019-51887-2.

PubMed ID: 31659210

Sasaki M, Nishikawa H, Suzuki S, Moser M, Huber M, Sawasato K, Matsubayashi HT, Kumazaki K, Tsukazaki T, Kuruma Y, Nureki O, Ueda T, Nishiyama KI. The bacterial protein YidC accelerates MPIase-dependent integration of membrane proteins. J Biol Chem. 2019 Dec 6;294(49):18898-18908. doi: 10.1074/jbc.RA119.011248. Epub 2019 Oct 29.

PubMed ID: 31662434

Kumar S, Mahendran I, Athreya A, Ranjan R, Penmatsa A. Isolation and structural characterization of a Zn2+-bound single-domain antibody against NorC, a putative multidrug efflux transporter in bacteria. J Biol Chem. 2020 Jan 3;295(1):55-68. doi: 10.1074/jbc.RA119.010902. Epub 2019 Nov 7.

PubMed ID: 31699895

Monterroso B, Zorrilla S, Sobrinos-Sanguino M, Robles-Ramos MÁ, Alfonso C, Söderström B, Meiresonne NY, Verheul J, den Blaauwen T, Rivas G. The Bacterial DNA Binding Protein MatP Involved in Linking the Nucleoid Terminal Domain to the Divisome at Midcell Interacts with Lipid Membranes. MBio. 2019 May 28;10(3). pii: e00376-19. doi: 10.1128/mBio.00376-19.

PubMed ID: 31138739

Kortzak D, Alleva C, Weyand I, Ewers D, Zimmermann MI, Franzen A, Machtens JP, Fahlke C. Allosteric gate modulation confers K+ coupling in glutamate transporters. EMBO J. 2019 Oct 1;38(19):e101468. doi: 10.15252/embj.2019101468. Epub 2019 Sep 10.

PubMed ID: 31506973

Yoneshiro T, Wang Q, Tajima K, Matsushita M, Maki H, Igarashi K, Dai Z, White PJ, McGarrah RW, Ilkayeva OR, Deleye Y, Oguri Y, Kuroda M, Ikeda K, Li H, Ueno A, Ohishi M, Ishikawa T, Kim K, Chen Y, Sponton CH, Pradhan RN, Majd H, Greiner VJ, Yoneshiro M, Brown Z, Chondronikola M, Takahashi H, Goto T, Kawada T, Sidossis L, Szoka FC, McManus MT, Saito M, Soga 5, Kajimura S. BCAA catabolism in brown fat controls energy homeostasis through SLC25A44. Nature. 2019 Aug;572(7771):614-619. doi: 10.1038/s41586-019-1503-x. Epub 2019 Aug 21.

PubMed ID: 31435015

Girish V, Pazzi J, Li A, Subramaniam AB. Fabrics of Diverse Chemistries Promote the Formation of Giant Vesicles from Phospholipids and Amphiphilic Block Copolymers. Langmuir. 2019 Jul 16;35(28):9264-9273. doi: 10.1021/acs.langmuir.9b01621. Epub 2019 Jul 5.

PubMed ID: 31276413

Leano JB, Batarni S, Eriksen J, Juge N, Pak JE, Kimura-Someya T, Robles-Colmenares Y, Moriyama Y, Stroud RM, Edwards RH. Structures suggest a mechanism for energy coupling by a family of organic anion transporters. PLoS Biol. 2019 May 13;17(5):e3000260. doi: 10.1371/journal.pbio.3000260. eCollection 2019 May.

PubMed ID: 31083648

Okazaki KI, Wöhlert D, Warnau J, Jung H, Yildiz Ö, Kühlbrandt W, Hummer G. Mechanism of the electroneutral sodium/proton antiporter PaNhaP from transition-path shooting. Nat Commun. 2019 Apr 15;10(1):1742. doi: 10.1038/s41467-019-09739-0.

PubMed ID: 30988359

Mosslehy W, Voskoboynikova N, Colbasevici A, Ricke A, Klose D, Klare JP, Mulkidjanian AY, Steinhoff HJ. Conformational Dynamics of Sensory Rhodopsin II in Nanolipoprotein and Styrene-Maleic Acid Lipid Particles. Photochem Photobiol. 2019 Mar 8. doi: 10.1111/php.13096. [Epub ahead of print]

PubMed ID: 30849183

Catipovic MA, Bauer BW, Loparo JJ, Rapoport TA. Protein translocation by the SecA ATPase occurs by a power-stroke mechanism. EMBO J. 2019 Mar 15. pii: e101140. doi: 10.15252/embj.2018101140. [Epub ahead of print]

PubMed ID: 30877095

Owens TW, Taylor RJ, Pahil KS, Bertani BR, Ruiz N, Kruse AC, Kahne D. Structural basis of unidirectional export of lipopolysaccharide to the cell surface. Nature. 2019 Mar;567(7749):550-553. doi: 10.1038/s41586-019-1039-0. Epub 2019 Mar 20.

PubMed ID: 30894747

Brunner JD, Schenck S. Preparation of Proteoliposomes with Purified TMEM16 Protein for Accurate Measures of Lipid Scramblase Activity. Methods Mol Biol. 2019;1949:181-199. doi: 10.1007/978-1-4939-9136-5_14.

PubMed ID: 30790257

Anna Stikane , Ee Taek Hwang , Emma V. Ainsworth , Samuel Piper , Kevin Critchley , Julea Butt , Erwin reisner and Lars J C Jeuken. Towards Compartmentalized Photocatalysis: Multiheme Proteins as Transmembrane Molecular Electron Conduits. 2018 December 21. DOI: 10.1039/C8FD00163D (Paper) Faraday Discuss., 2018, Accepted Manuscript


Hannah M. Britt , Clara A. García-Herrero ORCID, Paul W. Denny ORCID, Jackie A. Mosely and John M. Sanderson. Lytic reactions of drugs with lipid membranes. DOI: 10.1039/C8SC04831B (Edge Article) Chem. Sci., 2019, 10, 674-680.


Berry T, Dutta D, Chen R, Leong A, Wang H, Donald WA, Parviz M, Cornell B, Willcox M, Kumar N, Cranfield CG. The lipid membrane interactions of the cationic antimicrobial peptide chimeras melimine and cys-melimine. Langmuir. 2018 Aug 17. doi: 10.1021/acs.langmuir.8b01701. [Epub ahead of print]

PubMed ID: 30119612

Fujikawa K, Suzuki S, Nagase R, Ikeda S, Mori S, Nomura K, Nishiyama KI, Shimamoto K. Syntheses and Activities of the Functional Structures of a Glycolipid Essential for Membrane Protein Integration. ACS Chem Biol. 2018 Aug 15. doi: 10.1021/acschembio.8b00654. [Epub ahead of print]

PubMed ID: 30064209

Peterson, E., T. Ryser, S. Funk, D. Inouye, M. Sharma, H. Qin, T.A. Cross, and D.D. Busath. (2011). Functional reconstitution of influenza A M2(22-62). Biochim Biophys Acta 1808:516-21.

PubMed ID: 20969830

Stuart, L.J., Buck, J.P., Tremblay, A.E., Buist, P.H. (2006) Configurational analysis of cyclopropyl fatty acids isolated from Escherichia coli. Org Lett. 2006 Jan 5;8(1):79-81.

PubMed ID: 16381572