SNAP-tag Reacting Lipids

SNAP-tag is a polypeptide with 182 residues that can be fused to proteins of interest. SNAP-tags are widely used in biochemistry to investigate the function and localization of proteins and enzymes. SNAP-tag was engineered to accept O-benzylguanine derivatives. Avanti’s SNAP-tag lipids are currently 18:1 PE-benzylguanine and 18:1 PE-PEG2000-benzylguanine making them SNAP-tag reactive. SNAP-tag molecules are used in many applications including RNA-editing, development of SNAP-based sensors for small molecules, and protein-DNA complexes.

Rosa Merlo, Diego Caprioglio, Michele Cillo, Anna Valenti, Rosanna Mattossovich, Castrese Morrone, Alberto Massarotti, Franca Rossi, Riccardo Miggiano, Antonio Leonardi, Alberto Minassi & Giuseppe Perugino (2021) The SNAP-tag technology revised: an effective chemo-enzymatic approach by using a universal azide-based substrate, Journal of Enzyme Inhibition and Medicinal Chemistry, 36:1, 85-97, DOI: 10.1080/14756366.2020.1841182