Research Spotlight: Deep Mining of Oxysterols and Cholestenoic Acids in Human Plasma and Cerebrospinal Fluid: Quantification using Isotope Dilution Mass Spectrometry

Posted on March 15, 2021


Oxysterol Splash Graphical Abstract

"Deep Mining of Oxysterols and Cholestenoic Acids in Human Plasma and Cerebrospinal Fluid: Quantification using Isotope Dilution Mass Spectrometry"

The analysis of human plasma/serum and cerebrospinal fluid (CSF) by mass spectrometry (MS) could prove to be particularly useful when looking for biomarkers of disease, particularly those related to cholesterol biosynthesis and metabolism and progression of neurodegeneration. Dr. Griffiths’ laboratory used Avanti’s oxysterolSPLASH to make quantitative measurements of the NIST SRM 1950 plasma sample.

It was determined that stable isotope dilution MS coupled with the use of authentic isotopically labeled standards was the most reliable method for sterol quantification. Using OxysterolSPLASH along with isotope labeled cholesterol, the study reports the absolute quantification of 17 sterols, the semi-quantification of eight sterols, and approximate quantification of one sterol in the absence of an available standard in a NIST SRM 1950 plasma sample. In addition to the quantified species, seven sterols were identified and another eight were and partially identified.

Along with analyzing the NIST SRM 1950 plasma sample, they explored the quantification of oxysterols in a CSF sample using the same OxysterolSPLASH standard mixture. With this new method for analysis, they were able to measure the levels of both C-24 and C-25 epimers; whereas, only the combined levels of the epimers had been previously quantified.

The absolute and semi-quantification of oxysterols and cholestanoic acids from this study were in agreement with other studies that have been conducted. The work presented by Dr. Griffiths and his team of collaborators continues to further advances of standarization within the lipidomics community. We are excited to know that our OxysterolSPLASH is making such an impact in the area of lipidomics, and we look forward to what will be accomplished next with our LIPIDOMIX Standards

Click HERE to read this study in its entirety and to learn more about absolute quantification vs semi-quantification vs approximate quantification.

Image Credit: Graphical Abstract from Original Research Publication