New Product Spotlight: Detergents

Posted on May 12, 2020


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In this month’s New Product Spotlight, we’re highlighting detergents! Keep reading for a list of some of Avanti’s unique detergents and how to utilize them in your research.

C11-Neopentyl Glycol Triglucoside (click here to view on our website)

This glucoside detergent is markedly effective at stabilizing many membrane proteins, including transporters and GPCRs. The presence of the glucoside head group is expected to facilitate the formation of small protein-detergent complexes, or PDCs, that are ideal for the determination of protein structures. Not to mention that this compound’s mild properties also make it perfect for extracting protein without aggregation or denaturation effects.

Meso-Butanetetraol-Based Maltoside-C11 (click here to view on our website)

This dimaltoside utilizes the stereo-chemistry of the detergent molecule to effectively stabilize proteins, including several transporters and GPCRs. This is the first detergent developed to do this. It contains a symmetrical plane, which is thought to be ideally associated with the crystal lattice formation of membrane proteins.—plus it can be used as an alternative to DDM and LMNG.

Vitamin-Based Glycoside-3 (click here to view on our website)

This detergent is effective at stabilizing diverse membrane proteins, as well as providing long-term stability for both GPCR & GPCR-G protein complexes. Also amenable to membrane protein study via the CryoEM method, this compound gives clear visualization of protein complexes with multiple subunits.

Dendritic Trimaltoside-A6 (click here to view on our website)

This detergent is nonionic, and is specifically effective in the stabilization and visualization of GPCRs. It’s the first detergent made up of a dendronic hydrophobic group. Human beta2AR and its G protein complex were stabilized by using this agent, which is notable. This resulted in monodisperse particles and clear visualization of the complex via CryoEM.

Resorcinarene-Based Amphiphile-C11 (click here to view on our website)

As a mild glucoside detergent, this can be used as an alternative to GDN, digitonin, and CHAPS detergent. Because of the formation of small protein-detergent complexes, or PDCs, with GPCRs, along with its enhanced stabilizing effect, this detergent is ideal for the determination of protein structures—particularly for CryoEM-based structural study.

Tandem Malonate Glucoside-A13 (click here to view on our website)

This glucoside is effective for stabilizing diverse membrane proteins including GCPRs. Because it has a high tendency to form small protein-detergent complexes, or PDCs, this is a great tool for protein structural studies using X-ray crystallography and CryoEM. This is also an excellent alternative to OGNG because of the markedly enhanced protein stabilization efficiency compared to OGNG.

Mesitylene-Cored Glucoside Amphiphile-C13 (click here to view on our website)

Designed for protein crystallization studies, this glucoside displays a marked behavior for the stabilization of diverse membrane proteins—including transporters and GPCRs. The presence of the glucoside head group facilitates the formation of small complexes with membrane proteins, which is beneficial for high quality protein crystal formation and the clear visualization via CryoEM.

Meta-Xylene-Linked Glucose Amphiphile C12 (click here to view on our website)

Remarkably, this glucoside is more effective than LMNG or DDM at stabilizing GPCRs. The main favorable feature of this agent? The glucoside head group, which imparts marked protein stabilization efficacy—particularly for GPCRs.

Meta-Xylene-Linked Maltose Amphiphile C6.8 (click here to view on our website)

A maltoside version of Meta-Xylene-Linked Glucose Amphiphile C12 (above), this detergent can be used in detergent-free buffers with little protein aggregation. Its alkyl chain contains a branch point that increases interaction with membrane proteins allowing the use of this amphiphile in detergent-free buffers, with very little protein aggregation. This compound’s ability to stabilize versatile membrane proteins, particularly for GPCRs, is notable.

Norbornane-Based Maltoside-C11 (click here to view on our website)

Composed of a rigid bicyclic ring in the central region, this research-grade detergent is one of the best agents for GPCR stability. This detergent is expected to facilitate protein crystallization and protein stabilization better than LMNG because of the molecule’s high rigidity—this makes it an excellent candidate for GPCR structural studies.

See any products that spark your interest? Click here for ordering information! For a complete list of Avanti’s detergents, click here.