LNP Applications Highlight: Insight into Theranostic Nanovesicles Prepared by Thin Lipid Hydration and Microfluidic Method

Posted on August 06, 2021


Microfluidics Vs Tlh

Lipid nanoparticle formation can be achieved via several methods including mechanical methods, replacement of organic solvents with aqueous media, and detergent removal methods. The most common method for the formation of nanoparticle liposomes involves lipid film formation and hydration of the film with an aqueous buffer. Microfluidics has been gaining attention as a method for nanoparticle preparation due to its reportedly robust, scalable, and reproducible nature. The microfludic preparation methodology allows accurate nanoparticle production by controlling the flow rate ratio, total flow rate, and the organic solvent and lipid concentrations. Particles produced via microfluidic methodology typically exhibit a smaller size, higher drug loading, and a narrower size distribution. Formulating a lipid nanoparticle is a major part of developing controlled, targeted lipid delivery systems for therapeutics. But once these nanoparticles have been formulated the battle isn’t over. The scalability and wide use of the system is only as good as its manufacturing method. So, let’s take a look at some research that might help you decide the best way to manufacture your lipid nanoparticles!

A theranostic liposome comprised of a lipid derivative of an MRI contrast agent and a model photosensitizer, zinc phthalocyanine, for photodynamic therapy was prepared via the thin lipid film hydration (TLH) method. The researchers from this article proposed a study that compared the TLH prepared theranostic liposomes to ones prepared via microfluidics.

The research found that microfluidics does offer several advantages for preparation of theranostic lipid nanoparticles. The zinc phthalocyanine photosensitizer was shown to incorporate significantly better via microfluidics than with the TLH method. The photosensitizer was loaded at 2-3 times the loading capacity found in lipid nanoparticles prepared via TLH. The lipid nanoparticles prepared via microfluidics were also found to have improved relaxation which possibly improves the MRI contrast and reduces the administered dose of contrast agent. The research also suggests that a high surface area to volume ratio and a lower PPC to zinc phthalocyanine photosensitizer ratio could lead to increased diffusion of water molecules through the lipid nanoparticles membrane and enable interactions with paramagnetic centers. While the overall performance of the lipid nanoparticles was unchanged when switching from the TLH preparation method to microfluidics, the research did show several potential manufacturing advantages that microfluidics offers over typical TLH manufacturing.

If you’re interested in using Avanti’s lipids or formulations expertise in your theranostic lipid nanoparticle research visit our website today! We are leaders in lipid nanoparticle technology and can help you at any step of the process!


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