Research Spotlight

Optimized assay for lipid normalization in LC-MS/MS applications

Posted on January 19, 2023

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The volume of lipidomics research has exploded in the past decade. Lipids are increasingly being recognized as major players in health and disease, and as such, quantifying lipids in cells has become increasingly more important. There are many methods for normalizing lipid concentrations in quantitative lipidomics studies, but to date there is not a clear consensus for which is best. Dr. Kari Basso, Mass Spectrometry Research and Education Center at the University of Florida, recently published an exciting article optimizing the sulfo-phospho-vanillin assay (SPVA) for lipid normalization in LC-MS/MS applications. Keep reading for more!

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Lipids in Small-Molecule Pro-Drugs

Posted on October 24, 2022

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Lipids are used in a variety of ways in therapeutics. Recently, lipids have garnered a lot of attention for their role in mRNA delivery, but that's not all they're good for. At least four types of lipids are being investigated as pro-moieties in pro-drugs - fatty acids, glycerides, steroids, and phospholipids. Keep reading to learn more!

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Lipids in Action: Gravity Defying Geckos

Posted on October 21, 2022

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Lipids are abundant in nature and important in many different ways. Our focus is usually placed on their functions in human biology and therapeutic applications, but this research spotlight is a little different. Keep reading to find out how lipids make geckos the climbing creatures they are!

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Ceramides and Clinical Depression: How They're Linked

Posted on September 28, 2022

October 3-10 is Mental Illness Awareness Week! To help raise awareness and fight the stigma associated with mental illness we want to share some exciting research. Dive into this research spotlight to find out how ceramides are linked to clinical depression and how future treatments could take advantage of this link.

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Novel Homeostatic Mechanism Tunes PI(4,5)P2-dependent Signaling at the PM

Posted on July 20, 2022

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This research spotlight features research conducted by our Junior Lipid Leader Rachel Wills. Rachel, along with the rest of Dr. Hammond's lab at the University of Pittsburgh, is interested in phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) and its regulation in mammalian plasma membrane. In this spotlight, Rachel and Dr. Hammond elucidate a novel mechanism by which PI(4,5)P2 is regulated. This insight can be used in the future to find potential therapeutic targets for diseases resulting from genetic defects of PI(4,5)P2 synthesis. Keep reading to learn more about this novel mechanism!

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Capturing the Ceramide Complexity of the Stratum Corneum

Posted on July 14, 2022

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Atopic dermatitis and psoriasis are caused by changes in ceramide levels in the skin. Understanding the complexity and identifying the exact ceramide components in the skin is important to work towards cures and treatments for these skin diseases. Researchers at Hokaido University used a recently developed method involving the combination of multiple reaction monitoring (MRM) mode of LC-MS/MS with Avanti's ceramide standards to identify 400 species of ceramides in the stratum cornuem. Keep reading to see what they found!

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Could antibiotic resistance be linked to the lipidome?

Posted on June 16, 2022

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The CDC’s Antibiotic Resistance Threats in the United States, 2019 presented data showing more than 2.8 million antibiotic-resistant infections occur annually in the US. And of these 2.8 million infections, more than 35,000 people died as a result. The emergence and spread of new antibiotic-resistant forms is alarming. Investing in the development of new vaccines, improving existing therapeutics, and discovering better ways of diagnosing infections caused by these pathogens hinge on one underlying principle – understanding the pathogens themselves. Enter Dr. Hines and her team at UGA! Read the full article to find out how they are researching the lipidome of Staphylococcus Aureus to better understand its antibiotic resistance.

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Could An Injection One Day Protect You From Stress and Stress-Related Depression?

Posted on May 09, 2022

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Stress – most of us experience it in some way every day. Some stress is good, but the longer we experience stress, the more taxed our physiological stress response becomes until it is taxed beyond its capabilities. Chronic stress not only taxes our stress response but can also lead to other health problems such as depression. Research conducted in 2021 suggests that treating depression at the source is a viable treatment option. Studies recently identified lipopolysaccharide (LPS) as a possible treatment for depression in chronically stressed mice. LPS has several adverse side effects, but Avanti offers Phosphorylated Hexaacyl Disaccharide (PHAD) which could be a safe and effective alternative! Check out the full article to learn more.

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Using Polymer-Lipid Nanodiscs to Better Understand Alzheimer's Disease

Posted on April 18, 2022

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Our Lipid Leader Research Spotlight Series highlights recent research conducted by leaders in the field. This month, Dr. Ayyalusamy Ramamoorthy was kind enough to join us as our Lipid Leader. Dr. Rams works to develop new polymer lipid-nanodiscs and uses these nanodiscs to investigate biochemical and biophysical properties of membrane proteins and amyloidogenic proteins. Check out this spotlight to learn more about why they are developing new polymers for these nanodiscs and how they are using them to better understand neurodegenerative disease such as Alzheimer's Disease!

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Combatting the Opioid Crisis with Liposomal Adjuvant Systems

Posted on April 05, 2022

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Headlines across the globe have largely been dominated by the COVID-19 pandemic for the last two-plus years – and rightly so. But infectious diseases such as COVID-19 are not the only public health crises that we face. Heart disease, cancer, and diabetes affect millions of lives every day, and we hear about the research being done to combat them regularly. In this research spotlight, let’s take a look at a public health crisis that we don’t focus on as much – the opioid addiction crisis.

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Lipidome Variability Within Subjects Leads to Novel Insights Into Specific Coronary Plaque Phenotypes

Posted on March 04, 2022

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Since the early 2000s, lipidomic analysis has become an increasingly hot research topic. The development of newer, better mass spectrometry technologies and standardized lipids and lipid mixtures continues to allow the lipidomics research community to get closer and closer to their goal – clinical translation of lipidomic research for the development of diagnostic and therapeutic tools. Avanti is a leader in the development of standardized lipids and lipid mixtures used in lipidomics research. We continue to develop our offerings to align with the needs of the research community. Federico Torta, a dear customer and leader in the lipidomic analysis community, recently published research linking visit-to-visit variability of certain lipid classes to the burden of coronary atherosclerosis in humans.

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Investigating New Treatment Options for Triple Negative Breast Cancer

Posted on February 28, 2022

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Triple negative breast cancer (TNBC) accounts for 10-15% of all breast cancers. TNBC is a particularly dangerous type of cancer due to its rapid growth and spreading, but also because of limited treatment options currently available. TNBC lacks estrogen and progesterone receptors which means that hormone therapy is not a viable treatment option. And the absence of high levels of HER2 protein means that HER2 targeted drugs are not a treatment option either. Thus, the “triple negative” designation for this cancer, and the reason that treatment options are currently limited to mainly chemotherapies. Finding a better treatment option for TNBC is imperative.

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Research Spotlight: AdipoAtlas: A reference lipidome for human white adipose tissue

Posted on February 03, 2022

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Since 1975, worldwide obesity has nearly tripled. As of 2016, almost 2 billion adults are overweight, and about one-third of those are obese. Even children under the age of five are becoming obese at an alarming rate. At least 2.8 million people die every year as a result of being overweight or obese. How can we discover biomarkers for obesity? Keep reading to learn what our February Lipid Leader, Dr. Maria Fedorova, and her team at TU-Dresden uncovered!

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Research Spotlight: PMP2/FABP8 Induces PI(4,5)P2-Dependent Transbilayer Reorganization of Sphingomyelin in the Plasma Membrane

Posted on December 14, 2021

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We love to read research results published by our valued customers, and we love to share those results with you! Toshihide Kobayashi, National Center for Scientific Research/University of Strasbourg, said this about our lipids “We cannot publish these results without Avanti lipids. Thank you very much for the high-quality lipids.” Thank you, Toshihide, for being a loyal Avanti customer! Okay, let’s dive into the fantastic research that Toshihide has been doing! Keep reading to learn more!

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Research Spotlight: Combinatorial Liposomal Peptide Vaccine Induces IgA and Confers Protection Against Influenza Virus and Bacterial Super-Infection

Posted on November 12, 2021

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3D-PHAD… sounds cool, right? Well, there’s more to this Avanti Lipid product than a cool name. Avanti’s PHAD is the first fully synthetic monophosphoryl lipid A (MPLA) available for use in human vaccines. But what does the 3D stand for? 3D means that this PHAD product is a 3-deacylated form of MPLA. Avanti’s PHAD products are great adjuvants for human vaccine applications due to their ability to elicit a strong immune response while also exhibiting a lower pyrogenic profile than the naturally occurring bacterial agent. Enough about this product’s name, keep reading to learn how it’s being used!

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Research Spotlight: A Potent Cancer Vaccine Adjuvant System for Particleization of Short, Synthetic CD8+ T Cell Epitopes

Posted on October 29, 2021

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To kill cancer cells, short-tumor-derived peptides (8-10 amino acids in length) must be recognized by the CD8+ T cell receptors. This promising method for killing cancer cells has not produced the desired results in clinical studies. Keep reading to learn how researchers at the University of Buffalo are working to improve this method!

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Research Spotlight: Prophylactic efficacy against Mycobacterium tuberculosis using ID93 and lipid-based adjuvant formulations in the mouse model

Posted on October 22, 2021

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Globally, tuberculosis, a treatable and preventable disease, kills 1.6 million people every year. A latent form of tuberculosis, Mycobacterium tuberculosis (Mtb), is estimated to infect nearly a quarter of the world’s total population. It is imperative that we as a scientific community find alternatives to the current TB vaccines being used today. Keep reading to learn more about a research team doing just that!

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Research Spotlight: An Improved and Highly Selective Fluorescence Assay for Measuring Phosphatidylserine Decarboxylase Activity

Posted on October 05, 2021

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Hopefully, you’ve had the opportunity to read our interview with Dr. Dennis Voelker. If you haven’t, SPOILER ALERT! In the interview, Dr. Voelker describes the significance that a recently developed fluorescence assay has in future diagnostic and therapeutic research. He says “The field of lipid biochemistry is ripe for expansion into the realm of screening for- and identifying- novel inhibitors of essential enzymes for application as new drugs for treating all types of microbial infections, including viruses, bacteria and fungi.” Keep reading to learn more about this highly selective fluorescence assay for measuring phosphatidylserine decarboxylase activity!

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Research Spotlight: World Brain Day!

Posted on July 07, 2021

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World Brain Day is July 22, 2021! Keep reading to learn about research, from one of our former lipid leaders, aimed at identifying metabolites that contribute to neurodegeneration resulting from Cerebrotendinous xanthomatosis (CTX)!

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Research Spotlight: 1-Stearoyl-2-docosahexaenoyl-phosphatidic acid interacts with and activates Praja-1, the E3 ubiquitin ligase acting on the serotonin transporter in the brain

Posted on July 07, 2021

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We hope you had a chance to read our most recent Lipid Leaders Interview with Dr. Fumio Sakane. To follow that great interview, we wanted to share one of his recent publications investigating the importance of lipid composition on the regulation of serotonin levels and how this contributes to the pathophysiology/therapeutics of various psychiatric disorders such as OCD, depression, autism, and schizophrenia. Keep reading to learn more!

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Research Spotlight: The Acyl Chains of Phosphoinositide PIP3 Alter the Structure and Function of Nuclear Receptor Steroidogenic Factor-1

Posted on June 23, 2021

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Nuclear receptor steroidogenic factor-1 (SF-1, NR5A1) specifically binds the signaling phosphoinositides, PI(4,5)P2 and PI(3,4,5)P3. Research has previously shown how the headgroups of these PIP lipids interact with and regulate SF-1 function. But what role do the acyl chains of these PIPs play in this interaction? Keep reading to see what researchers at Vanderbilt University School of Medicine found out.

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Research Spotlight: Cardiolipin-Induced Activation of Pyruvate Dehydrogenase Links Mitochondrial Lipid Biosynthesis to TCA Cycle Function

Posted on June 10, 2021

Hopefully, you’ve had time to read our interview with Dr. Miriam Greenberg, but if you haven’t it’s a MUST read! If you’re wondering what type of research she has been engaged in recently, keep reading this review of an article she published in 2019 titled “Cardiolipin-Induced Activation of Pyruvate Dehydrogenase Links Mitochondrial Lipid Biosynthesis to TCA Cycle Function”

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Research Spotlight: C24:0 and C24:1 Sphingolipids in Cholesterol-Containing, Five- and Six-Component Lipid Membranes

Posted on May 12, 2021

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We hope you had a chance to read our most recent Lipid Leaders Interview with Dr. Felix Goni. In the interview, Dr. Goni gave us some great insight into his research at the University of the Basque Country and Instituto Biofisika. To follow that, we wanted to share one of his recent publications investigating C24:0 and C24:1 Sphingolipids in Cholesterol-Containing, Five- and Six-Component Lipid Membranes. Dr. Goni has been a loyal Avanti customer and friend for many years, and it is always exciting to see what his group accomplishes! Keep reading to learn more!

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Research Spotlight: Sex Differences in Insulin Sensitivity are Related to Muscle Tissue Acylcarnitine But Not Subcellular Lipid Distribution

Posted on April 26, 2021

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Our first May Research Spotlight uses Avanti's Lipidomic Standards to investigate lipid subspecies distribution as a contributor to differences in insulin sensitivity between men and women with obesity. Keep reading to check out the great work going on at University of Colorado Anschutz Medical Campus in Aurora, Colorado!

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Research Spotlight: Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines

Posted on April 12, 2021

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We hope you had a chance to read our most recent Lipid Leaders Interview with Dr. Yvonne Perrie. In the interview, Dr. Perrie gave us some great insight into her current research. To follow that, we wanted to share one of her recent publications investigating the impact of different delivery systems on the efficacy of self-amplifying mRNA (SAM) vaccines. Dr. Perrie and her team prepared and compared four different cationic SAM delivery platforms including liposomes, solid lipid nanoparticles (SLNs), polymeric nanoparticles (NPs), and emulsions. These lipid-based delivery platforms were prepared using Avanti’s DOTAP and DDA products. Keep reading to find out what this investigation uncovered!

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Research Spotlight: Lipopolymers and Lipids from Lung Surfactants in Association with N-acetyl-l-cysteine: Characterization and Cytotoxicity

Posted on March 30, 2021

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Our first April Research Spotlight comes from Nadia Chiaramoni. Nadia connected with us on Twitter and shared one of her recent works where she utilized our diacetylene lipids to form polymeric liposomes as a possible N-acetyl-I-cysteine (NAC) delivery system. Keep reading to check out Nadia’s publication in the Chemistry and Physics of Lipids journal and the great work that she is doing at the Universidad Nacional de Quilmes in Buenos Aires, Argentina.

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Research Spotlight: Deep Mining of Oxysterols and Cholestenoic Acids in Human Plasma and Cerebrospinal Fluid: Quantification using Isotope Dilution Mass Spectrometry

Posted on March 15, 2021

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Our second citation promotion for March comes from Dr. William Griffiths at Swansea University Medical School in Wales. Dr. Griffiths and his team of collaborators used Avanti’s oxysterolSPLASH and individual oxysterol standards to develop methodology to identify and quantify multiple sterols (oxysterols and sterol-acids) in plasma and cerebrospinal fluid. Avanti’s own, Lisa Connell, Director of the Lipidomics Division, is included as an author on the paper for her work in developing these standards. Keep reading to learn more about this exciting work!

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Research Spotlight: Mono- and Poly-unsaturated Phosphatidic Acid Regulates Distinct Steps of Regulated Exocytoses in Neuroendocrine Cells

Posted on February 16, 2021

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We would like to thank Nick Vitale from the Universite de Strasbourg in France for submitting this publication to the Citation Promotion Contest! Nick and his team are loyal customers of Avanti, and it’s exciting to see what they have been able to accomplish in the work titled “Mono- and Poly-Unsaturated Phosphatidic Acid Regulates Distinct Steps of Regulated Exocytoses in Neuroendocrine Cells.” Keep reading for more!

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Research Spotlight: Using Avanti LIPIDOMIX® Standards to Create Lipidomics Software

Posted on February 01, 2021

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As we continue our Citation Promotion series, check out how Jeremy Koelmel and his research team use Avanti LIPIDOMIX® Standards to create lipidomics software for the semi-quantification of lipids! They have released the LipidMatch Flow, LipidMatch Normalizer, and Lipid Annotator software platforms. Keep reading to find out how to access their software and how you can use it for analysis in your lipidomics research!

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Research Spotlight: Localization of Cyclopropane Modifications in Bacterial Lipids via 213 nm Ultraviolet Photodissociation Mass Spectrometry

Posted on January 21, 2021

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Check out this exciting research submitted, as part of our Citation Promotion, by Molly Blevins from the Department of Chemistry, University of Texas at Austin! The work is titled “Localization of Cyclopropane Modifications in Bacterial Lipids via 213 nm Ultraviolet Photodissociation Mass Spectrometry”. Molly and the rest of the Brodbelt research group were able to employ 213 nm ultraviolet photodissociation mass spectrometry (UVPD-MS) for structural characterization of cyclopropane rings in bacterial phospholipids and mycolic acids.

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