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Equipment

Avanti Mini Extruder allows researchers and scientists to prepare large, unilamellar vesicles by extrusion in an efficient manner.

Avanti Mini Extruder

Convenient

  • Prepare large, unilameller vesicles (LUVs) by extrusion in an easy, rapid, and efficient manner.
  • Design allows for the rapid cleaning of all wetted parts, reducing “down-time” between production of vesicles from different lipid species
  • Generate uniform populations of unilamellar liposomes without the use of solvents or detergents
  • Vesicle size readily controlled by polycarbonate membrane selection

Affordable

The Avanti Mini-Extruder is constructed of stainless steel and Teflon; the new design is guaranteed to give years of trouble-free service and is available for a fraction of the cost of a larger extruder.

  • Available for a fraction of the cost of a larger extruder
  • All parts/replacement parts are available from Avanti
  • Complete set includes:
    • Mini Extruder
    • Heating Block
    • 2 O-Rings
    • 2 Gas Tight syringes
    • 100 Polycarbonate Membranes
    • 100 Filter supports

Flexible

Placing the stabilizer block-extruder assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.

  • Optional heating block allows the extrusion of vesicles at elevated temperatures, which is critical for the successful production of vesicles from phospholipids with a phase transition temperature above room temperature
  • Generate unilamellar liposomes ranging from 30nm-1 µm when assembled with appropriate polycarbonate membrane
  • Useful for a wide variety of lipid composition
  • Interchangeable with 250 µL and 1 mL syringes

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Reliable

The particle size distribution of unilamellar vesicles prepared by extrusion is a function of the number of passes through the extruder membrane.

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Hydrated lipid solutions will initially form large, multilamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids.

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Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.

Products

Instructions

  • Assembly – detailed instructions on assembly of the Avanti Mini-Extruder
  • Extrusion – detailed instructions on extrusion of liposomes
  • Cleaning – detailed instructions on cleaning of syringes

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