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Home Lipidomics Antibodies
E06 mAb
E06 Mouse Monoclonal Antibody (IgM)
Anti-(Oxidized Phospholipid)
330001

330001S
100 μg $200
All Prices in US Dollars.

Description


The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized LDL, oxidized HDL and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in mice, zebrafish, rabbits, monkeys and humans. It also binds to apoptotic cells, but not viable cells, and can be used in a variety of applications, including ELISA, immunohistochemistry and Western blot analysis, as well as for in vivo imaging with MRI techniques.

Avanti now provides high purity E06 from animal-free culture of hybridoma cells. Each batch is
verified for activity and provided with detailed protocols for the above applications.

The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

References


Tsimikas, S., Aikawa, M., Miller, F.J., Jr., Miller, E.R., Torzewski, M., Lentz, S.R., Bergmark, C., Heistad, D.D., Libby, P., Witztum, J.L. (2007) Increased plasma oxidized phospholipid:apolipoprotein B-100 ratio with concomitant depletion of oxidized phospholipids from atherosclerotic lesions after dietary lipid-lowering: a potential biomarker of early atherosclerosis regression. Arterioscler Thromb Vasc Biol.  27:175-81 [PubMed]

Qin, J., Goswami, R., Balabanov, R., Dawson, G. (2007) Oxidized phosphatidylcholine is a marker for neuroinflammation in multiple sclerosis brain. J Neurosci Res. 85:977-84. [PubMed]
 
Friedman, P., Hörkkö, S., Steinberg, D., Witztum, J.L., Dennis, E.A. (2002) Correlation of antiphospholipid antibody recognition with the structure of synthetic oxidized phospholipids. Importance of Schiff base formation and aldol condensation. J Biol Chem. 277:7010-20. [PubMed]

Hörkkö, S., Bird, D.A., Miller, E., Itabe, H., Leitinger, N., Subbanagounder, G., Berliner, J.A., Friedman, P., Dennis, E.A., Curtiss, L.K., Palinski, W., Witztum, J.L. (1999) Monoclonal autoantibodies specific for oxidized phospholipids or oxidized phospholipid-protein adducts inhibit macrophage uptake of oxidized low-density lipoproteins.
J Clin Invest. 103:117-28. [PubMed]

Palinski, W., Hörkkö, S., Miller, E., Steinbrecher, U.P., Powell, H.C., Curtiss, L.K., Witztum, J.L. (1996) Cloning of monoclonal autoantibodies to epitopes of oxidized lipoproteins from apolipoprotein E-deficient mice. Demonstration of epitopes of oxidized low density lipoprotein in human plasma. J Clin Invest. 98: 800-14. [PubMed]

Antibody Information


Antigen:
Oxidized LDL
Ig Class:
Mouse IgM
Specificity:
E06 recognizes the phosphocholine headgroup of oxidized phospholipid that is present in oxidized LDL and PC-modified BSA. E06 does not bind to normal LDL or unoxidized PC.
Antibody Source:
Monoclonal antibody from C57BL/6-derived hybridoma E06.
Production:
In vitro cell culture.
Purification:
Ultra filtration through 100 KDa cut-off filters.
Purity:
≥ 95%.
Formulation:
E06 is provided as a 0.2μm sterile-filtered solution
in phosphate buffered saline (PBS).
Recommended
Applications:
ELISA (Sandwich, Direct & Competitive), IHC and WB (see Technical Data Sheet under Downloads tab for more information).
Storage conditions:
Store undiluted at 4°C or less.
Hazardous/Non-hazardous Components:
This product contains no substances that, at their given concentration, are known to be hazardous to health. Therefore there is no MSDS for this product.

Antibody for Atherosclerosis Research

There is a growing body of evidence that oxidized lipids, particularly oxidized phospholipids (OxPL), play a crucial role in the development and pathology of inflammatory diseases and some infectious diseases. Atherosclerosis is widely considered to be a chronic inflammatory disease and elevated plasma LDL is a major risk factor. Since oxidized LDL (OxLDL), with its associated OxPL, plays a major role in atherogenesis, there has been a need for antibodies that specifically recognize OxLDL. The E06 monoclonal antibody meets this need and can discriminate between native LDL and OxLDL by binding to the phosphocholine headgroup of OxPL that is present in OxLDL but is absent from native LDL. In addition, E06 can detect OxPL in cells, tissues, membranes and lipoproteins in a variety of inflammatory settings.

 

E06 was extensively characterized in the laboratory of Dr. Joseph Witztum at the University of California at San Diego (UCSD). Avanti® Polar Lipids, Inc. is now producing the E06 murine monoclonal antibody under license from UCSD.



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